RRC ID 21926
Author Wang Z, Wilhelmsson C, Hyrsl P, Loof TG, Dobes P, Klupp M, Loseva O, Mörgelin M, Iklé J, Cripps RM, Herwald H, Theopold U.
Title Pathogen entrapment by transglutaminase--a conserved early innate immune mechanism.
Journal PLoS Pathog.
Abstract Clotting systems are required in almost all animals to prevent loss of body fluids after injury. Here, we show that despite the risks associated with its systemic activation, clotting is a hitherto little appreciated branch of the immune system. We compared clotting of human blood and insect hemolymph to study the best-conserved component of clotting systems, namely the Drosophila enzyme transglutaminase and its vertebrate homologue Factor XIIIa. Using labelled artificial substrates we observe that transglutaminase activity from both Drosophila hemolymph and human blood accumulates on microbial surfaces, leading to their sequestration into the clot. Using both a human and a natural insect pathogen we provide functional proof for an immune function for transglutaminase (TG). Drosophila larvae with reduced TG levels show increased mortality after septic injury. The same larvae are also more susceptible to a natural infection involving entomopathogenic nematodes and their symbiotic bacteria while neither phagocytosis, phenoloxidase or-as previously shown-the Toll or imd pathway contribute to immunity. These results firmly establish the hemolymph/blood clot as an important effector of early innate immunity, which helps to prevent septic infections. These findings will help to guide further strategies to reduce the damaging effects of clotting and enhance its beneficial contribution to immune reactions.
Volume 6(2)
Pages e1000763
Published 2010-2-12
DOI 10.1371/journal.ppat.1000763
PMID 20169185
PMC PMC2820530
MeSH Animals Bacterial Infections / immunology* Blood Coagulation / immunology* Drosophila / immunology Drosophila Proteins / immunology Hemolymph / immunology* Hemolymph / microbiology Humans Immunity, Innate* Microscopy, Fluorescence Sepsis / immunology Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Transglutaminases / immunology*
IF 6.463
Times Cited 84
WOS Category MICROBIOLOGY PARASITOLOGY VIROLOGY
Resource
Drosophila 7356R-2