Reference - Detail
RRC ID | 21945 |
---|---|
Author | Osman D, Buchon N, Chakrabarti S, Huang YT, Su WC, Poidevin M, Tsai YC, Lemaitre B. |
Title | Autocrine and paracrine unpaired signaling regulate intestinal stem cell maintenance and division. |
Journal | J Cell Sci |
Abstract |
The Janus kinase (JAK) signal transducer and activator of transcription (STAT) pathway is involved in the regulation of intestinal stem cell (ISC) activity to ensure a continuous renewal of the adult Drosophila midgut. Three ligands, Unpaired 1, Unpaired 2 and Unpaired 3 (Upd1, Upd2 and Upd3, respectively) are known to activate the JAK/STAT pathway in Drosophila. Using newly generated upd mutants and cell-specific RNAi, we showed that Upd1 is required throughout the fly life to maintain basal turnover of the midgut epithelium by controlling ISC maintenance in an autocrine manner. A role of Upd2 and Upd3 in basal conditions is discernible only in old gut, where they contribute to increased ISC abnormal division. Finally, upon an acute stress such as oral bacterial infection, we showed that Upd3 is released from enterocytes and has an additive effect with Upd2 to promote rapid epithelial regeneration. Taken together, our results show that Upd ligands are required to maintain the midgut homeostasis under both normal and pathological states. |
Volume | 125(Pt 24) |
Pages | 5944-9 |
Published | 2012-12-15 |
DOI | 10.1242/jcs.113100 |
PII | jcs.113100 |
PMID | 23038775 |
MeSH | Animals Autocrine Communication Cell Differentiation / physiology Cell Division Cell Growth Processes / physiology Drosophila / cytology* Drosophila / genetics Drosophila / metabolism Drosophila / microbiology Drosophila Proteins / biosynthesis Drosophila Proteins / genetics Female Intestinal Mucosa / metabolism Intestines / cytology* Intestines / microbiology Janus Kinases / metabolism Paracrine Communication Pectobacterium carotovorum / physiology STAT Transcription Factors / metabolism Stem Cells / cytology* Transcription Factors / biosynthesis Transcription Factors / genetics |
IF | 4.573 |
Times Cited | 71 |
WOS Category | CELL BIOLOGY |
Resource | |
Drosophila |