論文 - 詳細
| RRC ID | 21963 |
|---|---|
| 著者 | Ray P, Luo X, Rao EJ, Basha A, Woodruff EA 3rd, Wu JY. |
| タイトル | The splicing factor Prp31 is essential for photoreceptor development in Drosophila. |
| ジャーナル | Protein Cell |
| Abstract |
Retinitis pigmentosa is a leading cause of blindness and a progressive retinal disorder, affecting millions of people worldwide. This disease is characterized by photoreceptor degeneration, eventually leading to complete blindness. Autosomal dominant (adRP) has been associated with mutations in at least four ubiquitously expressed genes encoding pre-mRNA splicing factors-Prp3, Prp8, Prp31 and PAP1. Biological function of adRP-associated splicing factor genes and molecular mechanisms by which mutations in these genes cause cell-type specific photoreceptor degeneration in humans remain to be elucidated. To investigate the in vivo function of these adRP-associated splicing factor genes, we examined Drosophila in which expression of fly Prp31 homolog was down-regulated. Sequence analyses show that CG6876 is the likely candidate of Drosophila melanogaster Prp31 homolog (DmPrp31). Predicted peptide sequence for CG6876 shows 57% similarity to the Homo sapiens Prp31 protein (HsPrp31). Reduction of the endogenous Prp31 by RNAi-mediated knockdown specifically in the eye leads to reduction of eye size or complete absence of eyes with remarkable features of photoreceptor degeneration and recapitulates the bimodal expressivity of human Prp31 mutations in adRP patients. Such transgenic DmPrp31RNAi flies provide a useful tool for identifying genetic modifiers or interacting genes for Prp31. Expression of the human Prp31 in these animals leads to a partial rescue of the eye phenotype. Our results indicate that the Drosophila CG6876 is the fly ortholog of mammalian Prp31 gene. |
| 巻・号 | 1(3) |
| ページ | 267-74 |
| 公開日 | 2010-3-1 |
| DOI | 10.1007/s13238-010-0035-9 |
| PMID | 21203973 |
| PMC | PMC4875087 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Base Sequence DNA Primers / genetics Drosophila Proteins / antagonists & inhibitors Drosophila Proteins / genetics Drosophila Proteins / physiology* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / physiology Eye Abnormalities / genetics Eye Proteins / antagonists & inhibitors Eye Proteins / genetics Eye Proteins / physiology* Gene Knockdown Techniques Genes, Insect Humans Molecular Sequence Data Pancreatitis-Associated Proteins Photoreceptor Cells, Invertebrate / physiology* RNA Interference RNA Splicing Sequence Homology, Amino Acid |
| IF | 10.164 |
| 引用数 | 2 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 6876R-1 6876R-4 |