Author Hirata T, Osuga Y, Yoshino O, Hirota Y, Harada M, Takemura Y, Morimoto C, Koga K, Yano T, Tsutsumi O, Taketani Y.
Title Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.
Journal Hum Reprod
Abstract BACKGROUND:Aiming at improving an animal model of endometriosis, we developed a homologous mouse model using 'green mice' that ubiquitously express green fluorescent protein.
METHODS:Endometrial fragment obtained from estradiol (E2)-supplemented ovariectomized 'green mice' was minced and injected into the peritoneal cavity of ovariectomized wild-type mice. The recipient wild mice were raised with or without E2 supplementation for 2 weeks, and then were euthanized. Endometriotic lesions that developed in the abdomen were examined both macroscopically and microscopically under fluorescence, and weight of the lesions was measured.
RESULTS:The endometriotic lesions were more clearly detected under fluorescence imaging than by conventional macroscopic examination. Histologically, endometriotic lesions deriving from 'green mice' were sharply distinguished from surrounding host tissues under fluorescence microscopy. More lesions developed in E2-supplemented than control recipient mice. The measured fluorescence intensity of endometriotic lesions showed significant positive correlation with their weight (R=0.844, P<0.0001), and was significantly higher in E2-supplemented mice than in vehicle-supplemented mice (P=0.0062).
CONCLUSION:The present endometriosis model using 'green mice' would be useful for expeditious identification and quantitative evaluation of endometriotic lesions.
Volume 20(8)
Pages 2092-6
Published 2005-8-1
DOI 10.1093/humrep/dei012
PII dei012
PMID 15831509
MeSH Animals Disease Models, Animal* Endometriosis / pathology Endometriosis / physiopathology* Estradiol / pharmacology Female Green Fluorescent Proteins / genetics* Mice Mice, Inbred C57BL Mice, Transgenic* Organ Size Ovariectomy
IF 5.506
Times Cited 52
Mice C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb