論文 - 詳細
| RRC ID | 2210 |
|---|---|
| 著者 | Nakagawa T, Inagaki A, Ito T, Fujimura S, Miyaji T, Yurimoto H, Kato N, Sakai Y, Tomizuka N. |
| タイトル | Regulation of two distinct alcohol oxidase promoters in the methylotrophic yeast Pichia methanolica. |
| ジャーナル | Yeast |
| Abstract |
In this study, two Pichia methanolica alcohol oxidase (AOD) promoters, P(MOD1) and P(MOD2), were evaluated in a promoter assay system utilizing the acid phosphatase (AP) gene from Saccharomyces cerevisiae (ScPHO5) as a reporter. Heterologous gene expression driven by the P(MOD1) and P(MOD2) promoters was found to be strong and tightly regulated by carbon source at the transcriptional level. P(MOD1) was induced not only by methanol but also by glycerol. P(MOD2) was induced only by methanol, although it was not repressed on the addition of glycerol to a methanol medium, suggesting that P(MOD2) is regulated in a manner distinct from that of other AOD-gene promoters. On the other hand, methanol and oxygen level-influenced gene expression mediated by P(MOD1) and P(MOD2). P(MOD1) expression was optimal at low methanol concentrations, whereas P(MOD2) was predominantly expressed at high methanol and high oxygen concentrations. Based on these results, both P(MOD2) and P(MOD1) should be useful tools for controlling heterologous gene expression in P. methanolica. In particular, it should be possible to differentially control the production phases of two heterologous proteins, using P(MOD1) and P(MOD2) in the same host cell and in the same flask. |
| 巻・号 | 23(1) |
| ページ | 15-22 |
| 公開日 | 2006-1-15 |
| DOI | 10.1002/yea.1334 |
| PMID | 16411161 |
| MeSH | Acid Phosphatase / genetics Acid Phosphatase / metabolism Alcohol Oxidoreductases / genetics* Alcohol Oxidoreductases / metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Histocytochemistry Methanol / metabolism Mutagenesis, Insertional Oxygen / metabolism Pichia / genetics* Pichia / metabolism* Promoter Regions, Genetic / genetics* |
| IF | 3.143 |
| 引用数 | 17 |
| WOS 分野 | MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | ?ScPHO5 gene |