Reference - Detail
| RRC ID | 2230 |
|---|---|
| Author | Shimoda C, Itadani A, Sugino A, Furusawa M. |
| Title | Isolation of thermotolerant mutants by using proofreading-deficient DNA polymerase delta as an effective mutator in Saccharomyces cerevisiae. |
| Journal | Genes Genet Syst |
| Abstract |
Eukaryotic DNA polymerases delta and epsilon, both of which are required for chromosomal DNA replication, contain proofreading 3'-->5'exonuclease activity. DNA polymerases lacking proofreading activity act as strong mutators. Here we report isolation of thermotolerant mutants by using a proofreading-deficient DNA polymerase delta variant encoded by pol3-01 in the yeast Saccharomyces cerevisiae. The parental pol3-01 strain grew only poorly at temperatures higher than 38 degrees C. By stepwise elevation of the incubation temperature, thermotolerant mutants that could proliferate at 40 degrees C were successfully obtained; however, no such mutants were isolated with the isogenic POL3 strain. The recessive hot1-1 mutation was defined by genetic analysis of a weak thermotolerant mutant. Strong thermotolerance to 40 degrees C was attained by multiple mutations, at least one of which was recessive. These results indicate that a proofreading-deficient DNA delta polymerase variant is an effective mutator for obtaining yeast mutants that have gained useful characteristics, such as the ability to proliferate in harsh environments. |
| Volume | 81(6) |
| Pages | 391-7 |
| Published | 2006-12-1 |
| DOI | 10.1266/ggs.81.391 |
| PII | JST.JSTAGE/ggs/81.391 |
| PMID | 17283384 |
| MeSH | DNA Polymerase III / genetics* Genes, Recessive Mutagenesis* Saccharomyces cerevisiae / genetics* Saccharomyces cerevisiae / growth & development Saccharomyces cerevisiae / physiology* Saccharomyces cerevisiae Proteins / genetics Temperature |
| IF | 0.917 |
| Times Cited | 26 |
| WOS Category | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Yeast | BY20479(AMY52-3D) BY20355(AMY128-1) |