| RRC ID |
2230
|
| Author |
Shimoda C, Itadani A, Sugino A, Furusawa M.
|
| Title |
Isolation of thermotolerant mutants by using proofreading-deficient DNA polymerase delta as an effective mutator in Saccharomyces cerevisiae.
|
| Journal |
Genes Genet Syst
|
| Abstract |
Eukaryotic DNA polymerases delta and epsilon, both of which are required for chromosomal DNA replication, contain proofreading 3'-->5'exonuclease activity. DNA polymerases lacking proofreading activity act as strong mutators. Here we report isolation of thermotolerant mutants by using a proofreading-deficient DNA polymerase delta variant encoded by pol3-01 in the yeast Saccharomyces cerevisiae. The parental pol3-01 strain grew only poorly at temperatures higher than 38 degrees C. By stepwise elevation of the incubation temperature, thermotolerant mutants that could proliferate at 40 degrees C were successfully obtained; however, no such mutants were isolated with the isogenic POL3 strain. The recessive hot1-1 mutation was defined by genetic analysis of a weak thermotolerant mutant. Strong thermotolerance to 40 degrees C was attained by multiple mutations, at least one of which was recessive. These results indicate that a proofreading-deficient DNA delta polymerase variant is an effective mutator for obtaining yeast mutants that have gained useful characteristics, such as the ability to proliferate in harsh environments.
|
| Volume |
81(6)
|
| Pages |
391-7
|
| Published |
2006-12-1
|
| DOI |
10.1266/ggs.81.391
|
| PII |
JST.JSTAGE/ggs/81.391
|
| PMID |
17283384
|
| MeSH |
DNA Polymerase III / genetics*
Genes, Recessive
Mutagenesis*
Saccharomyces cerevisiae / genetics*
Saccharomyces cerevisiae / growth & development
Saccharomyces cerevisiae / physiology*
Saccharomyces cerevisiae Proteins / genetics
Temperature
|
| IF |
0.917
|
| Times Cited |
26
|
|
WOS Category
|
GENETICS & HEREDITY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Yeast |
BY20479(AMY52-3D)
BY20355(AMY128-1) |
