RRC ID 2251
Author Motizuki M, Yokota S, Tsurugi K.
Title Effect of low pH on organization of the actin cytoskeleton in Saccharomyces cerevisiae.
Journal Biochim. Biophys. Acta
Abstract Cell growth in the yeast Saccharomyces cerevisiae depends on polarization of the actin cytoskeleton. In this study, we investigated how the cell regulates the distribution of actin in response to low pH conditions, focusing on the role of mitogen-activated protein kinases, Hog1 and Slt2. Changing the extracellular pH from 6.0 to 3.0 caused a transient depolarization of the actin cytoskeleton. Actin cables were no longer visible, and actin patches appeared randomly distributed after 30 min at pH 3.0. The deletion strain hog1Delta did not show this low-pH phenotype, suggesting that Hog1 is involved in depolarization of the actin cytoskeleton in response to low-pH stress. Yeast cells incubated at pH 3.0 also showed markedly increased endocytosis compared with the control at neutral pH, as indicated by the uptake of Lucifer Yellow (LY). Both the hog1Delta and slt2Delta mutants took up LY into the vacuole to a similar extent as the wild-type strain. In addition, cells grown at pH 3.0 showed a 2-fold increase in phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2) levels, as did the hog1Delta or slt2Delta cells. Efficient uptake of LY and actin repolarization at pH 3.0 might therefore require activation of PI(4,5)P2 synthesis.
Volume 1780(2)
Pages 179-84
Published 2008-2
DOI 10.1016/j.bbagen.2007.10.003
PII S0304-4165(07)00233-4
PMID 17980162
MeSH Actins / analysis Actins / metabolism* Cytoskeleton / chemistry Cytoskeleton / metabolism* Endocytosis Gene Deletion Hydrogen-Ion Concentration Isoquinolines / analysis Isoquinolines / metabolism Mitogen-Activated Protein Kinases / genetics Mitogen-Activated Protein Kinases / metabolism* Phosphatidylinositol 4,5-Diphosphate / metabolism Saccharomyces cerevisiae / growth & development* Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae / ultrastructure Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism*
IF 3.79
Times Cited 7
Yeast BY23752