RRC ID 2273
Author Sakakima Y, Hayashi S, Yagi Y, Hayakawa A, Tachibana K, Nakao A.
Title Gene therapy for hepatocellular carcinoma using sonoporation enhanced by contrast agents.
Journal Cancer Gene Ther
Abstract We examined whether sonoporation enhanced by a contrast agent (BR14) was effective in gene therapy for hepatocelluar carcinoma (HCC). Human hepatic cancer cells (SK-Hep1) and plasmid cDNAs expressing green fluorescent protein (GFP), interferonbeta (IFNbeta), and LacZ were used. In vitro, SK-Hep1 cell suspensions with DNA and BR14 were sonoporated. Expressions of every plasmid cDNA and the antitumor effect of IFNbeta were analyzed. In vivo, GFP and IFNbeta genes with BR14 were directly injected into subcutaneous tumors using SK-Hep1 in nude mice, and transcutaneous sonoporation of the tumors was performed. GFP gene transfections and tumor diameters after IFNbeta gene transfection were examined. In vitro, no SK-Hep1 cells were transfected without sonication, whereas transfections were successful after sonication with BR14. Antitumor effect of IFNbeta gene transfection by ultrasound (US) and with BR14 was revealed. In vivo, the SK-Hep1 cells expressed GFP, and the IFNbeta gene transfection by US with BR14 reduced tumor size significantly. In conclusion, gene therapy with sonoporation enhanced by a contrast agent may become a new treatment option for HCC.
Volume 12(11)
Pages 884-9
Published 2005-11-1
DOI 10.1038/sj.cgt.7700850
PII 7700850
PMID 15891773
MeSH Animals Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / therapy* DNA, Complementary / analysis Enzyme-Linked Immunosorbent Assay Female Fluorocarbons Genetic Therapy / methods* Green Fluorescent Proteins / analysis Humans Interferon-beta / analysis Liver Neoplasms / genetics Liver Neoplasms / therapy* Mice Mice, Inbred BALB C Mice, Nude Phospholipids Plasmids Transfection / methods* Tumor Cells, Cultured Ultrasonics*
IF 4.534
Times Cited 62
DNA material pCMV (Hu beta) (RDB01681) pCAZ 2 (RDB01870).