RRC ID |
22739
|
Author |
Banno Y, Nagasaki K, Tsukada M, Minohara Y, Banno J, Nishikawa K, Yamamoto K, Tamura K, Fujii T.
|
Title |
Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries.
|
Journal |
Cryobiology
|
Abstract |
Development of long-term preservation is essential for conservation of stocks of silkworm genetic resources. Thus far, a few methods have been reported, but more improvement is required for practical use. We have developed two effective modifications of a method for long-term preservation using frozen ovaries. One was slow cooling (1 °C per min) until -80 °C of the donor ovaries made possible by use of a BICELL freezing vessel. Using donor ovaries of 4th instar larvae, the average number of eggs laid per moth increased significantly from 110.7 ± 53.4 eggs per moth by slow cooling with the BICELL vessel vs 12.3 ± 10.3 eggs per moth by direct cooling in liquid nitrogen. A second improvement was connecting the thread bodies of the donor ovaries with those of the host in the transplantation step. Females operated on with the new method yielded a significantly higher percentage of moths that laid fertilized eggs than those transplanted with the standard procedure (70.4 ± 21.6% vs 22.9 ± 9.3%).
|
Volume |
66(3)
|
Pages |
283-7
|
Published |
2013-6-1
|
DOI |
10.1016/j.cryobiol.2013.03.004
|
PII |
S0011-2240(13)00111-9
|
PMID |
23500076
|
MeSH |
Animals
Bombyx / genetics
Bombyx / physiology*
Cryopreservation / methods
Cryopreservation / veterinary*
Female
Freezing
Larva / physiology
Ovary / physiology*
Ovary / transplantation*
Zygote / physiology
|
IF |
2.287
|
Times Cited |
6
|
WOS Category
|
BIOLOGY
PHYSIOLOGY
|
Resource |
Silkworms |
xe28
r03
p20
p50 |