Reference - Detail
| RRC ID | 22739 |
|---|---|
| Author | Banno Y, Nagasaki K, Tsukada M, Minohara Y, Banno J, Nishikawa K, Yamamoto K, Tamura K, Fujii T. |
| Title | Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries. |
| Journal | Cryobiology |
| Abstract |
Development of long-term preservation is essential for conservation of stocks of silkworm genetic resources. Thus far, a few methods have been reported, but more improvement is required for practical use. We have developed two effective modifications of a method for long-term preservation using frozen ovaries. One was slow cooling (1 °C per min) until -80 °C of the donor ovaries made possible by use of a BICELL freezing vessel. Using donor ovaries of 4th instar larvae, the average number of eggs laid per moth increased significantly from 110.7 ± 53.4 eggs per moth by slow cooling with the BICELL vessel vs 12.3 ± 10.3 eggs per moth by direct cooling in liquid nitrogen. A second improvement was connecting the thread bodies of the donor ovaries with those of the host in the transplantation step. Females operated on with the new method yielded a significantly higher percentage of moths that laid fertilized eggs than those transplanted with the standard procedure (70.4 ± 21.6% vs 22.9 ± 9.3%). |
| Volume | 66(3) |
| Pages | 283-7 |
| Published | 2013-6-1 |
| DOI | 10.1016/j.cryobiol.2013.03.004 |
| PII | S0011-2240(13)00111-9 |
| PMID | 23500076 |
| MeSH | Animals Bombyx / genetics Bombyx / physiology* Cryopreservation / methods Cryopreservation / veterinary* Female Freezing Larva / physiology Ovary / physiology* Ovary / transplantation* Zygote / physiology |
| IF | 2.287 |
| Times Cited | 6 |
| WOS Category | BIOLOGY PHYSIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Silkworms | xe28 r03 p20 p50 |