RRC ID 2276
Author Gründemann C, Bauer M, Schweier O, von Oppen N, Lässing U, Saudan P, Becker KF, Karp K, Hanke T, Bachmann MF, Pircher H.
Title Cutting edge: identification of E-cadherin as a ligand for the murine killer cell lectin-like receptor G1.
Journal J Immunol
Abstract The killer cell lectin-like receptor G1 (KLRG1) is expressed by NK cells and by T cells. In both humans and mice, KLRG1 identifies Ag-experienced T cells that are impaired in their proliferative capacity but are capable of performing effector functions. In this study, we identified E-cadherin as a ligand for murine KLRG1 by using fluorescently labeled, soluble tetrameric complexes of the extracellular domain of the murine KLRG1 molecule as staining reagents in expression cloning. Ectopic expression of E-cadherin in B16.BL6 target cells did not affect cell-mediated lysis by lymphokine-activated NK cells and by CD8 T cells but inhibited Ag-induced proliferation and induction of cytolytic activity of CD8 T cells. E-cadherin is expressed by normal epithelial cells, Langerhans cells, and keratinocytes and is usually down-regulated on metastatic cancer cells. KLRG1 ligation by E-cadherin in healthy tissue may thus exert an inhibitory effect on primed T cells.
Volume 176(3)
Pages 1311-5
Published 2006-2-1
DOI 10.4049/jimmunol.176.3.1311
PII 176/3/1311
PMID 16424155
MeSH Animals Antigens / immunology CD8-Positive T-Lymphocytes / cytology CD8-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / metabolism Cadherins / biosynthesis Cadherins / metabolism* Cadherins / physiology Cell Division / immunology Cell Line Growth Inhibitors / biosynthesis Growth Inhibitors / metabolism Growth Inhibitors / physiology Killer Cells, Lymphokine-Activated / immunology Killer Cells, Lymphokine-Activated / metabolism Ligands Melanoma, Experimental Mice Mice, Inbred C57BL Mice, Transgenic Receptors, Immunologic / genetics Receptors, Immunologic / metabolism*
IF 4.718
Times Cited 105
DNA material Mouse E-cadherin full length cDNA (RDB01184)