RRC ID |
22784
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著者 |
Lee JM, Kawakami N, Mon H, Mitsunobu H, Iiyama K, Ninaki S, Maenaka K, Park EY, Kusakabe T.
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タイトル |
Establishment of a Bombyx mori nucleopolyhedrovirus (BmNPV) hyper-sensitive cell line from the silkworm e21 strain.
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ジャーナル |
Biotechnol Lett
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Abstract |
Baculoviral expression systems, including those of Autographa californica multiple nucleopolyhedrovirus Bombyx mori nucleopolyhedrovirus (BmNPV), are used for recombinant protein production. Four B. mori-derived (BmN4, Bm5, Bmc140, and Bme21) cell lines were infected with recombinant BmNPV viruses expressing firefly luciferase or EGFP as reporters under the control of a viral polyhedrin promoter. Bme21 exhibited significantly higher (100-fold) luciferase activity than BmN4 and Bm5. With the EGFP reporter protein, Bme21 cells showed a marked increase in the ratio of EGFP-positive cells, reaching 90 % on day 4 post-infection, while Bm5 and BmN4 cells had a slow increase in the ratio of their EGFP-positive population. The viral titer in a supernatant of Bme21 cell culture increased faster than those of Bm5 and BmN4 cells. This susceptibility indicates that the Bme21 cell line is useful for large-scale protein expression using BmNPV.
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巻・号 |
34(10)
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ページ |
1773-9
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公開日 |
2012-10-1
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DOI |
10.1007/s10529-012-0971-y
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PMID |
22714272
|
MeSH |
Animals
Bombyx / genetics*
Bombyx / virology*
Cell Line
Genetic Vectors / genetics*
Green Fluorescent Proteins / chemistry
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Nucleopolyhedroviruses / genetics*
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics*
Recombinant Fusion Proteins / metabolism
Sf9 Cells
Transfection
|
IF |
1.977
|
引用数 |
12
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WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
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リソース情報 |
カイコ |
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