論文 - 詳細
| RRC ID | 22789 |
|---|---|
| 著者 | Masumoto M, Ohde T, Shiomi K, Yaginuma T, Niimi T. |
| タイトル | A Baculovirus immediate-early gene, ie1, promoter drives efficient expression of a transgene in both Drosophila melanogaster and Bombyx mori. |
| ジャーナル | PLoS One |
| Abstract |
Many promoters have been used to drive expression of heterologous transgenes in insects. One major obstacle in the study of non-model insects is the dearth of useful promoters for analysis of gene function. Here, we investigated whether the promoter of the immediate-early gene, ie1, from the Bombyx mori nucleopolyhedrovirus (BmNPV) could be used to drive efficient transgene expression in a wide variety of insects. We used a piggyBac-based vector with a 3xP3-DsRed transformation marker to generate a reporter construct; this construct was used to determine the expression patterns driven by the BmNPV ie1 promoter; we performed a detailed investigation of the promoter in transgene expression pattern in Drosophila melanogaster and in B. mori. Drosophila and Bombyx belong to different insect orders (Diptera and Lepidoptera, respectively); however, and to our surprise, ie1 promoter-driven expression was evident in several tissues (e.g., prothoracic gland, midgut, and tracheole) in both insects. Furthermore, in both species, the ie1 promoter drove expression of the reporter gene from a relatively early embryonic stage, and strong ubiquitous ie1 promoter-driven expression continued throughout the larval, pupal, and adult stages by surface observation. Therefore, we suggest that the ie1 promoter can be used as an efficient expression driver in a diverse range of insect species. |
| 巻・号 | 7(11) |
| ページ | e49323 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0049323 |
| PII | PONE-D-12-17137 |
| PMID | 23152896 |
| PMC | PMC3496687 |
| MeSH | Animals Animals, Genetically Modified Baculoviridae / genetics* Bombyx / embryology Bombyx / genetics* DNA / genetics Drosophila melanogaster / genetics* Embryo, Nonmammalian / metabolism Gene Expression* Genes, Immediate-Early / genetics* Genes, Reporter Genome, Insect / genetics Green Fluorescent Proteins / metabolism Larva / genetics Mutagenesis, Insertional / genetics Promoter Regions, Genetic* Transformation, Genetic Transgenes / genetics* |
| IF | 2.74 |
| 引用数 | 10 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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