| RRC ID |
22795
|
| 著者 |
Ishii K, Adachi T, Imamura K, Takano S, Usui K, Suzuki K, Hamamoto H, Watanabe T, Sekimizu K.
|
| タイトル |
Serratia marcescens induces apoptotic cell death in host immune cells via a lipopolysaccharide- and flagella-dependent mechanism.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Injection of Serratia marcescens into the blood (hemolymph) of the silkworm, Bombyx mori, induced the activation of c-Jun NH(2)-terminal kinase (JNK), followed by caspase activation and apoptosis of blood cells (hemocytes). This process impaired the innate immune response in which pathogen cell wall components, such as glucan, stimulate hemocytes, leading to the activation of insect cytokine paralytic peptide. S. marcescens induced apoptotic cell death of silkworm hemocytes and mouse peritoneal macrophages in vitro. We searched for S. marcescens transposon mutants with attenuated ability to induce apoptosis of silkworm hemocytes. Among the genes identified, disruption mutants of wecA (a gene involved in lipopolysaccharide O-antigen synthesis), and flhD and fliR (essential genes in flagella synthesis) showed reduced motility and impaired induction of mouse macrophage cell death. These findings suggest that S. marcescens induces apoptosis of host immune cells via lipopolysaccharide- and flagella-dependent motility, leading to the suppression of host innate immunity.
|
| 巻・号 |
287(43)
|
| ページ |
36582-92
|
| 公開日 |
2012-10-19
|
| DOI |
10.1074/jbc.M112.399667
|
| PII |
S0021-9258(20)62632-1
|
| PMID |
22859304
|
| PMC |
PMC3476324
|
| MeSH |
Animals
Apoptosis / immunology*
Bacterial Proteins / immunology
Bombyx / immunology*
Bombyx / microbiology
Flagella / immunology*
Hemocytes / immunology*
Hemocytes / microbiology
Immunity, Innate
Lipopolysaccharides / immunology*
Macrophages, Peritoneal / immunology*
Macrophages, Peritoneal / microbiology
Membrane Proteins / immunology
Mice
Serratia Infections / immunology*
Serratia Infections / microbiology
Serratia marcescens / immunology*
Serratia marcescens / metabolism
|
| IF |
4.238
|
| 引用数 |
25
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
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