RRC ID 22801
著者 Miyazaki S, Matsumoto Y, Sekimizu K, Kaito C.
タイトル Evaluation of Staphylococcus aureus virulence factors using a silkworm model.
ジャーナル FEMS Microbiol Lett
Abstract Previous studies have indicated that the silkworm model is useful for identifying virulence genes of Staphylococcus aureus, a human pathogenic bacterium. Here we examined the scope of S. aureus virulence factors that can be evaluated using the silkworm model. Gene-disrupted mutants of the agr locus, arlS gene and saeS gene, which regulate the expression of cell surface adhesins and hemolysins, exhibited attenuated virulence in silkworms. Mutants of the hla gene encoding α-hemolysin, the hlb gene encoding β-hemolysin, and the psmα and psmβ operons encoding cytolysins, however, showed virulence in silkworms indistinguishable from that of the parent strain. Thus, these S. aureus cytolysins are not required for virulence in silkworms. In contrast, the gene-disrupted mutants of clfB, fnbB and sdrC, which encode cell-wall-anchored proteins, attenuated S. aureus virulence in silkworms. In addition, the mutant of the srtA gene encoding sortase A, which anchors cell-wall proteins, showed attenuated virulence in silkworms. These findings suggest that the silkworm model can be used to evaluate S. aureus cell-wall proteins and regulatory proteins as virulence factors.
巻・号 326(2)
ページ 116-24
公開日 2012-1-1
DOI 10.1111/j.1574-6968.2011.02439.x
PMID 22092964
MeSH Animals Bombyx / microbiology* Disease Models, Animal* Gene Deletion Staphylococcus aureus / genetics Staphylococcus aureus / pathogenicity* Virulence Virulence Factors / genetics Virulence Factors / metabolism*
IF 1.987
引用数 35
WOS 分野 MICROBIOLOGY
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