RRC ID 22804
著者 Hanada Y, Sekimizu K, Kaito C.
タイトル Silkworm apolipophorin protein inhibits Staphylococcus aureus virulence.
ジャーナル J Biol Chem
Abstract Silkworm hemolymph inhibits hemolysin production by Staphylococcus aureus. We purified a factor in the silkworm hemolymph responsible for this inhibitory activity. The final fraction with the greatest specific activity contained 220- and 74-kDa proteins. Determination of the N-terminal amino acid sequence revealed that the 220- and 74-kDa proteins were apolipophorin I and apolipophorin II, respectively, indicating that the factor was apolipophorin (ApoLp). The purified ApoLp fraction showed decreased expression of S. aureus hla encoding α-hemolysin, hlb encoding β-hemolysin, saeRS, and RNAIII, which activate the expression of these hemolysin genes. Injection of an anti-ApoLp antibody into the hemolymph increased the sensitivity of silkworms to the lethal effect of S. aureus. Hog gastric mucin, a mammalian homologue of ApoLp, decreased the expression of S. aureus hla and hlb. These findings suggest that ApoLp in the silkworm hemolymph inhibits S. aureus virulence and contributes to defense against S. aureus infection and that its activity is conserved in mammalian mucin.
巻・号 286(45)
ページ 39360-9
公開日 2011-11-11
DOI 10.1074/jbc.M111.278416
PII S0021-9258(20)50594-2
PMID 21937431
PMC PMC3234760
MeSH Animals Apolipoproteins / genetics Apolipoproteins / metabolism Apolipoproteins / pharmacology* Bacterial Toxins / metabolism* Base Sequence Bombyx / genetics Bombyx / metabolism* Bombyx / microbiology Gene Expression Regulation, Bacterial / drug effects* Gene Expression Regulation, Bacterial / physiology Hemolysin Proteins / metabolism* Insect Proteins / genetics Insect Proteins / metabolism Insect Proteins / pharmacology* Molecular Sequence Data Staphylococcus aureus / metabolism* Staphylococcus aureus / pathogenicity*
IF 4.238
引用数 31
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
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