Reference - Detail
| RRC ID | 22812 |
|---|---|
| Author | Usui K, Miyazaki S, Kaito C, Sekimizu K. |
| Title | Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity. |
| Journal | Microb Pathog |
| Abstract |
Overnight cultures of 16 of 25 species of soil bacterium injected into silkworm hemolymph killed the silkworms. Injection of culture supernatants of five of these 16 bacterial species into the hemolymph also killed silkworms. Analysis of 16S rRNA indicated that the toxic species were Bacillus species. We purified an exotoxin produced by one of the Bacillus species by measuring the specific activity based on silkworm toxicity. The final step of purification revealed that the silkworm-killing activity was due to a 34-kDa protein. LC/MS/MS analysis revealed that the 34-kDa protein is sphingomyelinase C of Bacillus cereus. We propose that the silkworm infection model is useful for identifying pathogenic bacteria from soil and purifying bacterial toxins. |
| Volume | 46(2) |
| Pages | 59-62 |
| Published | 2009-2-1 |
| DOI | 10.1016/j.micpath.2008.10.010 |
| PII | S0882-4010(08)00137-X |
| PMID | 19041713 |
| MeSH | Animals Bacillus / classification Bacillus / genetics Bacillus / isolation & purification Bacillus / pathogenicity* Bacterial Toxins* / chemistry Bacterial Toxins* / isolation & purification Bacterial Toxins* / metabolism Bacterial Toxins* / toxicity Bacterial Typing Techniques Bombyx / microbiology* DNA, Bacterial / analysis Exotoxins* / chemistry Exotoxins* / isolation & purification Exotoxins* / metabolism Exotoxins* / toxicity Hemolymph / microbiology RNA, Ribosomal, 16S / genetics Soil Microbiology* Sphingomyelin Phosphodiesterase / metabolism |
| IF | 2.914 |
| Times Cited | 15 |
| WOS Category | MICROBIOLOGY IMMUNOLOGY |
| Resource | |
| Silkworms | |