論文 - 詳細
| RRC ID | 22814 |
|---|---|
| 著者 | Ono C, Kamagata T, Taka H, Sahara K, Asano S, Bando H. |
| タイトル | Phenotypic grouping of 141 BmNPVs lacking viral gene sequences. |
| ジャーナル | Virus Res |
| Abstract |
We constructed a series of gene knockout BmNPVs (KOVs) for each of 141 genes (Gomi et al., 1999; Katsuma et al., 2011) using the BmNPV T3 bacmid system (Ono et al., 2007) and lambda red recombination system (Datsenko and Wanner, 2000). In a subsequent analysis of the properties needed for infection using a marker gene, egfp (enhanced green fluorescent protein gene), inserted into the polyhedrin locus, the knockout viruses (KOVs) were subdivided into four phenotypic types, A to D. Type-A (86 KOVs) showed the ability to expand infections equivalent to the control while type-B (8 KOVs) spread infections more slowly. Type-C (37 KOVs) expressed egfp in transfected-BmN cells but the production of infectious viruses was not observed. Type-D (10 KOVs) showed no ability to express egfp even in the transfection experiments. KOVs lacking genes (pkip (Bm15), gp41 (Bm66), bro-d (Bm131), Bm20, 48, 65, 91, 93, or 101) previously identified as being essential, were placed in the viable type-A and B categories. |
| 巻・号 | 165(2) |
| ページ | 197-206 |
| 公開日 | 2012-5-1 |
| DOI | 10.1016/j.virusres.2012.02.016 |
| PII | S0168-1702(12)00064-0 |
| PMID | 22421381 |
| MeSH | Animals Bombyx Cell Line Gene Deletion* Genes, Reporter Genes, Viral* Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics Microbial Viability Nucleopolyhedroviruses / genetics Nucleopolyhedroviruses / physiology* Staining and Labeling / methods Virus Replication* |
| IF | 2.934 |
| 引用数 | 62 |
| WOS 分野 | VIROLOGY |
| オルトメトリクス指標 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 2.0 |
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