| RRC ID |
22819
|
| Author |
Kojima K, Oritani K, Nakatsukasa T, Asano S, Sahara K, Bando H.
|
| Title |
Ecdysone response element in a baculovirus immediate-early gene, ie1, promoter.
|
| Journal |
Virus Res
|
| Abstract |
A computer-assisted analysis identified tentative target sequences for regulatory proteins including ecdysone-inducible factors such as BmFTZ-F1 and Broad-Complex Z4 (BR-C Z4) in the ie1 promoter of BmNPV. A transient expression experiment using BmN cells and a series of truncated ie1 promoter constructs demonstrated that the activity of the ie1 promoter responded to alpha-ecdysone and 20-hydroxyecdysone, which required a tridecameric nucleotide stretch (ie1EcRE, 5'-GTGTTATCGACCT-3') homologous to the ecdysone response element reported for Drosophila (DmEcRE). RT-PCR demonstrated the expression of BmEcR and BmUSP, which are required as ecdysone-specific activators for EcRE-mediated activation, in BmN cells. Furthermore, the ie1 EcRE-mediated response was confirmed by using a recombinant BmNPV possessing a luciferase gene under the control of the ie1 promoter with or without ie1 EcRE. This is the first report of an ecdysone response element in a baculoviral gene promoter. These results also suggested that the regulation of the ie1 by ecdysone may militate viral replication at least under certain conditions during natural infections in vivo.
|
| Volume |
130(1-2)
|
| Pages |
202-9
|
| Published |
2007-12-1
|
| DOI |
10.1016/j.virusres.2007.06.012
|
| PII |
S0168-1702(07)00226-2
|
| PMID |
17658648
|
| MeSH |
Animals
Artificial Gene Fusion
Baculoviridae / genetics*
Baculoviridae / physiology
Bombyx
Cell Line
Ecdysone / metabolism*
Ecdysterone / metabolism
Gene Expression Regulation
Genes, Immediate-Early*
Genes, Reporter
Luciferases / biosynthesis
Luciferases / genetics
Promoter Regions, Genetic*
Response Elements / genetics*
|
| IF |
2.934
|
| Times Cited |
4
|
|
WOS Category
|
VIROLOGY
|
| Resource |
| Silkworms |
|
