Reference - Detail
| RRC ID | 22819 |
|---|---|
| Author | Kojima K, Oritani K, Nakatsukasa T, Asano S, Sahara K, Bando H. |
| Title | Ecdysone response element in a baculovirus immediate-early gene, ie1, promoter. |
| Journal | Virus Res |
| Abstract |
A computer-assisted analysis identified tentative target sequences for regulatory proteins including ecdysone-inducible factors such as BmFTZ-F1 and Broad-Complex Z4 (BR-C Z4) in the ie1 promoter of BmNPV. A transient expression experiment using BmN cells and a series of truncated ie1 promoter constructs demonstrated that the activity of the ie1 promoter responded to alpha-ecdysone and 20-hydroxyecdysone, which required a tridecameric nucleotide stretch (ie1EcRE, 5'-GTGTTATCGACCT-3') homologous to the ecdysone response element reported for Drosophila (DmEcRE). RT-PCR demonstrated the expression of BmEcR and BmUSP, which are required as ecdysone-specific activators for EcRE-mediated activation, in BmN cells. Furthermore, the ie1 EcRE-mediated response was confirmed by using a recombinant BmNPV possessing a luciferase gene under the control of the ie1 promoter with or without ie1 EcRE. This is the first report of an ecdysone response element in a baculoviral gene promoter. These results also suggested that the regulation of the ie1 by ecdysone may militate viral replication at least under certain conditions during natural infections in vivo. |
| Volume | 130(1-2) |
| Pages | 202-9 |
| Published | 2007-12-1 |
| DOI | 10.1016/j.virusres.2007.06.012 |
| PII | S0168-1702(07)00226-2 |
| PMID | 17658648 |
| MeSH | Animals Artificial Gene Fusion Baculoviridae / genetics* Baculoviridae / physiology Bombyx Cell Line Ecdysone / metabolism* Ecdysterone / metabolism Gene Expression Regulation Genes, Immediate-Early* Genes, Reporter Luciferases / biosynthesis Luciferases / genetics Promoter Regions, Genetic* Response Elements / genetics* |
| IF | 2.934 |
| Times Cited | 4 |
| WOS Category | VIROLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
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| Silkworms | |