| RRC ID |
22824
|
| 著者 |
Yokoyama H, Yokoyama T, Yuasa M, Fujimoto H, Sakudoh T, Honda N, Fugo H, Tsuchida K.
|
| タイトル |
Lipid transfer particle from the silkworm, Bombyx mori, is a novel member of the apoB/large lipid transfer protein family.
|
| ジャーナル |
J Lipid Res
|
| Abstract |
Lipid transfer particle (LTP) is a high-molecular-weight, very high-density lipoprotein known to catalyze the transfer of lipids between a variety of lipoproteins, including both insects and vertebrates. Studying the biosynthesis and regulation pathways of LTP in detail has not been possible due to a lack of information regarding the apoproteins. Here, we sequenced the cDNA and deduced amino acid sequences for three apoproteins of LTP from the silkworm (Bombyx mori). The three subunit proteins of the LTP are coded by two genes, apoLTP-II/I and apoLTP-III. ApoLTP-I and apoLTP-II are predicted to be generated by posttranslational cleavage of the precursor protein, apoLTP-II/I. Clusters of amphipathic secondary structure within apoLTP-II/I are similar to Homo sapiens apolipoprotein B (apoB) and insect lipophorins. The apoLTP-II/I gene is a novel member of the apoB/large lipid transfer protein gene family. ApoLTP-III has a putative conserved juvenile hormone-binding protein superfamily domain. Expression of apoLTP-II/I and apoLTP-III genes was synchronized and both genes were primarily expressed in the fat body at the stage corresponding to increased lipid transport needs. We are now in a position to study in detail the physiological role of LTP and its biosynthesis and assembly.
|
| 巻・号 |
54(9)
|
| ページ |
2379-90
|
| 公開日 |
2013-9-1
|
| DOI |
10.1194/jlr.M037093
|
| PII |
S0022-2275(20)35128-2
|
| PMID |
23812557
|
| PMC |
PMC3735936
|
| MeSH |
Amino Acid Sequence
Animals
Apolipoproteins B / genetics*
Bombyx / genetics*
Bombyx / growth & development
Bombyx / metabolism
Carrier Proteins / chemistry
Carrier Proteins / genetics*
Carrier Proteins / isolation & purification
Carrier Proteins / metabolism*
Cloning, Molecular
DNA, Complementary / genetics
Gene Expression Regulation, Developmental
Glycosylation
Humans
Insect Proteins / chemistry
Insect Proteins / genetics*
Insect Proteins / isolation & purification
Insect Proteins / metabolism*
Larva / genetics
Larva / metabolism
Molecular Sequence Data
Molecular Weight
Organ Specificity
Phylogeny
Sequence Homology
|
| IF |
4.483
|
| 引用数 |
9
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
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