| RRC ID |
2364
|
| 著者 |
Suzuki K, Tanaka I, Nakanishi I, Kurematsu A, Yakumaru H, Ikota N, Ishihara H.
|
| タイトル |
Drastic effect of several caffeic acid derivatives on the induction of heme oxygenase-1 expression revealed by quantitative real-time RT-PCR.
|
| ジャーナル |
Biofactors
|
| Abstract |
Among antioxidative polyphenols, caffeic acid esters such as caffeic acid phenethyl ester (CAPE) and chlorogenic acid are contained in propolis, vegetables and coffee. In this study, we compared the efficacy of some polyphenols on the activation level of a cytoprotective heme oxygenase-1 (HO-1) gene in RAW264.7 mouse macrophage cells using quantitative real-time RT-PCR. The quantitative study revealed a variety of activation level of HO-1 gene by the chemicals. CAPE and caffeic acid ethyl ester (CAEE) at the final concentration of 2 muM drastically activated the HO-1 gene to 39.2-fold and 20.1-fold, respectively. Curcumin, structurally related with caffeic acid and an element of turmeric, induced the HO-1 gene to 5.8-fold. In contrast, no activation was observed by other caffeic acid esters such as chlorogenic acid and rosmarinic acid. Higher concentrations were necessary for the activation by an antioxidant cysteamine and the electrophile diethyl maleate. Although the inducible activities of CAPE and chlorogenic acid were distinctly different, they showed similar reductive capacities when determined by cyclic voltammetry. These results show that the drastic activation of HO-1 gene by CAPE and CAEE is dependent upon their chemical structures, rather than the reductive activity of polyphenols, possibly reflecting the physiological effects of the nutritional elements.
|
| 巻・号 |
28(3-4)
|
| ページ |
151-8
|
| 公開日 |
2006-1-1
|
| DOI |
10.1002/biof.5520280301
|
| PMID |
17473375
|
| MeSH |
Animals
Caffeic Acids / pharmacology*
Cell Line
Cell Line, Tumor
Enzyme Induction / drug effects
Heme Oxygenase-1 / biosynthesis*
Humans
Mice
Phenylethyl Alcohol / analogs & derivatives*
Phenylethyl Alcohol / pharmacology
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
4.734
|
| 引用数 |
17
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
J774.1(RCB0434)
Hep G2 |
