RRC ID 2375
Author Unno Y, Okubo K, Wasaki J, Shinano T, Osaki M.
Title Plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of Lupin analysed by phytate utilization ability.
Journal Environ. Microbiol.
Abstract In the rhizosphere, phosphorus (P) levels are low because of P uptake into the roots. Rhizobacteria live on carbon (C) exuded from roots, and may contribute to plant nutrition by liberating P from organic compounds such as phytates. We isolated over 300 phytate (Na-inositol hexa-phosphate; Na-IHP)-utilizing bacterial strains from the rhizosheath and the rhizoplane of Lupinus albus (L.). Almost all of the isolates were classified as Burkholderia based on 16S rDNA sequence analysis. Rhizosheath isolates cultured with Na-IHP as the only source of C and P showed lower P uptake at the same extracellular phytase activity than rhizoplane strains, suggesting that bacteria from the rhizosheath utilized phytate as a C source. Many isolates also utilized insoluble phytate (Al-IHP and/or Fe-IHP). In co-culture with Lotus japonicus seedlings, some isolates promoted plant growth significantly.
Volume 7(3)
Pages 396-404
Published 2005-3
DOI 10.1111/j.1462-2920.2004.00701.x
PMID 15683400
MeSH Burkholderia / classification Burkholderia / enzymology Burkholderia / genetics Burkholderia / metabolism* Carbon / metabolism DNA, Bacterial / genetics Inositol Phosphates / metabolism* Phosphorus / metabolism* Phytic Acid / metabolism* Plant Roots / genetics Plant Roots / microbiology* Plant Roots / physiology RNA, Ribosomal, 16S / analysis RNA, Ribosomal, 16S / genetics Soil Microbiology*
IF 5.147
Times Cited 57
Lotus / Glycine Gifu B-129