論文 - 詳細
| RRC ID | 2377 |
|---|---|
| 著者 | Akashi T, Koshimizu S, Aoki T, Ayabe S. |
| タイトル | Identification of cDNAs encoding pterocarpan reductase involved in isoflavan phytoalexin biosynthesis in Lotus japonicus by EST mining. |
| ジャーナル | FEBS Lett |
| Abstract |
Isoflavans and pterocarpans are the major biosynthetically connected phytoalexins in legumes. A search of the expressed sequence tag library of a model legume Lotus japonicus, which produces an (-)-isoflavan, for homologs of phenylcoumaran benzylic ether reductase catalyzing the reductive cleavage of dihydrofurans, yielded seven full-length cDNAs, and the encoded proteins were analyzed in vitro. Four of them cleaved the dihydrofuran of a pterocarpan medicarpin to yield an isoflavan (-)-vestitol and were designated pterocarpan reductase (PTR). Two PTRs displayed enantiospecificity to (-)-medicarpin, representing genuine L. japonicus PTRs, while the other two lacked enantiospecificity and were presumed to be evolutionarily primitive types. |
| 巻・号 | 580(24) |
| ページ | 5666-70 |
| 公開日 | 2006-10-16 |
| DOI | 10.1016/j.febslet.2006.09.016 |
| PII | S0014-5793(06)01100-8 |
| PMID | 16996502 |
| MeSH | DNA, Complementary / genetics* DNA, Complementary / isolation & purification Databases, Nucleic Acid Expressed Sequence Tags Flavonoids / biosynthesis* Flavonoids / chemistry Glutathione / metabolism Lotus / enzymology* Lotus / genetics* Molecular Sequence Data Molecular Structure Oxidation-Reduction Oxidoreductases / metabolism* Phylogeny Pterocarpans / chemistry Pterocarpans / metabolism* Seedlings / enzymology Sesquiterpenes Terpenes / chemistry Terpenes / metabolism* |
| IF | 3.057 |
| 引用数 | 29 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | Gifu B-129 MWM249g01 MWM058d07 SPDL089e08 MPD075a04 MWM134b04 MWM092h09 MWM027h09) |