| RRC ID |
2377
|
| Author |
Akashi T, Koshimizu S, Aoki T, Ayabe S.
|
| Title |
Identification of cDNAs encoding pterocarpan reductase involved in isoflavan phytoalexin biosynthesis in Lotus japonicus by EST mining.
|
| Journal |
FEBS Lett
|
| Abstract |
Isoflavans and pterocarpans are the major biosynthetically connected phytoalexins in legumes. A search of the expressed sequence tag library of a model legume Lotus japonicus, which produces an (-)-isoflavan, for homologs of phenylcoumaran benzylic ether reductase catalyzing the reductive cleavage of dihydrofurans, yielded seven full-length cDNAs, and the encoded proteins were analyzed in vitro. Four of them cleaved the dihydrofuran of a pterocarpan medicarpin to yield an isoflavan (-)-vestitol and were designated pterocarpan reductase (PTR). Two PTRs displayed enantiospecificity to (-)-medicarpin, representing genuine L. japonicus PTRs, while the other two lacked enantiospecificity and were presumed to be evolutionarily primitive types.
|
| Volume |
580(24)
|
| Pages |
5666-70
|
| Published |
2006-10-16
|
| DOI |
10.1016/j.febslet.2006.09.016
|
| PII |
S0014-5793(06)01100-8
|
| PMID |
16996502
|
| MeSH |
DNA, Complementary / genetics*
DNA, Complementary / isolation & purification
Databases, Nucleic Acid
Expressed Sequence Tags
Flavonoids / biosynthesis*
Flavonoids / chemistry
Glutathione / metabolism
Lotus / enzymology*
Lotus / genetics*
Molecular Sequence Data
Molecular Structure
Oxidation-Reduction
Oxidoreductases / metabolism*
Phylogeny
Pterocarpans / chemistry
Pterocarpans / metabolism*
Seedlings / enzymology
Sesquiterpenes
Terpenes / chemistry
Terpenes / metabolism*
|
| IF |
3.057
|
| Times Cited |
29
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Lotus / Glycine |
Gifu B-129
MWM249g01
MWM058d07
SPDL089e08
MPD075a04
MWM134b04
MWM092h09
MWM027h09) |
