RRC ID 2399
Author Inoue K, Wakao H, Ogonuki N, Miki H, Seino K, Nambu-Wakao R, Noda S, Miyoshi H, Koseki H, Taniguchi M, Ogura A.
Title Generation of cloned mice by direct nuclear transfer from natural killer T cells.
Journal Curr. Biol.
Abstract Cloning mammals by nuclear transfer (NT) remains inefficient. One fundamental question is whether clones have really been derived from differentiated cells rather than from rare stem cells present in donor-cell samples. To date, cells, such as mature lymphocytes, with genetic differentiation markers have been cloned to generate mice only via a two-step NT involving embryonic stem (ES) cell generation and tetraploid complementation [1, 2 and 3]. Here, we show that the genome of a unique T-cell population, natural killer T (NKT) cells, can be fully reprogrammed by a single-step NT. The pups and their placentas possessed the rearranged TCR loci specific for NKT cells. The NKT-cell-cloned embryos had a high developmental potential in vitro: Most (71%) developed to the morula/blastocyst stage, in marked contrast to embryos from peripheral blood T cells (12%; p < 1 x 10(-25)). Furthermore, ES cell lines were efficiently established from these NKT-cell blastocysts. These findings clearly indicate a high level of plasticity in the NKT-cell genome. Thus, differentiation of the genome is not always a barrier to NT cloning for either reproductive or therapeutic purposes, so we can now postulate that at least some mammals cloned to date have indeed been derived from differentiated donor cells.
Volume 15(12)
Pages 1114-8
Published 2005-6-21
DOI 10.1016/j.cub.2005.05.021
PII S0960-9822(05)00508-7
PMID 15964276
MeSH Amino Acid Sequence Animals Base Sequence Blastocyst / physiology Cell Nucleus / genetics* Cloning, Organism / methods* Embryonic Development / genetics Female Gene Transfer Techniques Killer Cells, Natural / physiology* Male Mice Mice, Inbred Strains Molecular Sequence Data Receptors, Antigen, T-Cell / genetics
IF 9.251
Times Cited 97
Mice NKT clone mice 24-F5-2(RBRC01466) NKT clone mice 24-F4-1(RBRC01493)