| RRC ID |
2413
|
| Author |
Kumazawa M, Kobayashi M, Io F, Kawai T, Nishimura M, Ohno T, Horio F.
|
| Title |
Searching for genetic factors of fatty liver in SMXA-5 mice by quantitative trait loci analysis under a high-fat diet.
|
| Journal |
J Lipid Res
|
| Abstract |
Fatty liver is strongly associated with the metabolic syndrome characterized by obesity, insulin resistance, and type 2 diabetes, but the genetic basis and functional mechanisms linking fatty liver with the metabolic syndrome are largely unknown. The SMXA-5 mouse is one of the SMXA recombinant inbred substrains established from SM/J and A/J strains and is a model for polygenic type 2 diabetes, characterized by moderately impaired glucose tolerance, hyperinsulinemia, and mild obesity. SMXA-5 mice also developed fatty liver, and a high-fat diet markedly worsened this trait, although SM/J and A/J mice are resistant to fatty liver development under a high-fat diet. To dissect loci for fatty liver in the A/J regions of the SMXA-5 genome, we attempted quantitative trait loci (QTLs) analysis in (SM/JxSMXA-5)F2 intercross mice fed a high-fat diet. We mapped a major QTL for relative liver weight and liver lipid content near D12Mit270 on chromosome 12 and designated this QTL Fl1sa. The A/J allele at this locus contributes to the increase in these traits. We confirmed the effect of Fl1sa on lipid accumulation in liver using the A/J-Chr12(SM) consomic strain, which showed significantly less accumulation than A/J mice. This suggests that the SM/J and A/J strains, neither of which develops fatty liver, possess loci causing fatty liver and that the coexistence of these loci causes fatty liver in SMXA-5 mice.
|
| Volume |
48(9)
|
| Pages |
2039-46
|
| Published |
2007-9-1
|
| DOI |
10.1194/jlr.M700222-JLR200
|
| PII |
S0022-2275(20)42491-5
|
| PMID |
17595448
|
| MeSH |
Animals
Crosses, Genetic
Dietary Fats / administration & dosage*
Fatty Liver / genetics*
Female
Lod Score
Male
Mice
Mice, Inbred Strains
Microsatellite Repeats
Quantitative Trait Loci
|
| IF |
4.483
|
| Times Cited |
14
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Mice |
RBRC01221 |
