| RRC ID |
2435
|
| Author |
Hasegawa K, Fujioka T, Nakamura Y, Nakatsuji N, Suemori H.
|
| Title |
A method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation.
|
| Journal |
Stem Cells
|
| Abstract |
Human embryonic stem cells (hESCs) exhibit pluripotency and indefinite proliferation and are a potential source of cells for transplantation therapies and drug discovery. These applications will require large amounts of hESCs. However, hESCs are difficult to culture and maintain at larger scales, in part because of their low resistance to dissociation during passaging. To circumvent this, we developed a simple and easy method for establishing hESC sublines tolerant of complete dissociation. These cells exhibit high replating efficiency and also high cloning efficiency, and they maintain their ability to differentiate into the three germ layers. Several sublines have no detectable abnormalities in their karyotypes, and they retained their characteristics under feeder-free culture conditions and after freeze-thawing. Thus, these hESC sublines would be valuable for hESC applications.
|
| Volume |
24(12)
|
| Pages |
2649-60
|
| Published |
2006-12-1
|
| DOI |
10.1634/stemcells.2005-0657
|
| PII |
2005-0657
|
| PMID |
16931777
|
| MeSH |
Biomarkers
Cell Culture Techniques / methods*
Cell Differentiation / drug effects
Cell Line
Cell Separation / methods*
Chromosomes, Human / genetics
Clone Cells
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / drug effects
Growth Substances / pharmacology
Humans
Karyotyping
Polymorphism, Single Nucleotide / genetics
Wnt Proteins / pharmacology
|
| IF |
6.022
|
| Times Cited |
76
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
HEMATOLOGY
ONCOLOGY
CELL BIOLOGY
CELL & TISSUE ENGINEERING
|
| Resource |
| Human embryonic stem cells |
KhES-1
KhES-2
KhES-3 |
