Reference - Detail
| RRC ID | 2439 |
|---|---|
| Author | Hasegawa K, Cowan AB, Nakatsuji N, Suemori H. |
| Title | Efficient multicistronic expression of a transgene in human embryonic stem cells. |
| Journal | Stem Cells |
| Abstract |
The applicability of human embryonic stem cells (hESCs) will be greatly enhanced by techniques that permit efficient genetic modification with multiple transgenes. We report here on single-promoter-driven foot-and-mouth disease virus segment 2A-mediated multicistronic expression of a transgene in hESCs. Efficient multicistronic expression of the transgene was permitted by 2A-mediated separation with almost the same amounts of encoded proteins in hESC. In addition, the multicistronic protein expression was successful in hESC-derived differentiated cells in in vivo and in vitro differentiation assays. This technology may be a significant advance in the genetic engineering of hESCs and hESC-derived cells for purposes that require the reliable expression of multiple transgenes. Disclosure of potential conflicts of interest is found at the end of this article. |
| Volume | 25(7) |
| Pages | 1707-12 |
| Published | 2007-7-1 |
| DOI | 10.1634/stemcells.2006-0813 |
| PII | 2006-0813 |
| PMID | 17395772 |
| MeSH | Animals Embryonic Stem Cells / cytology Embryonic Stem Cells / metabolism* Foot-and-Mouth Disease Virus / genetics Gene Expression* Humans Mice Transgenes / genetics* Viral Proteins / genetics |
| IF | 6.022 |
| Times Cited | 59 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY ONCOLOGY HEMATOLOGY CELL & TISSUE ENGINEERING CELL BIOLOGY |
| Resource | |
| Human embryonic stem cells | KhES-1 KhES-2 KhES-3 |