| RRC ID |
2446
|
| 著者 |
Kim MJ, Liu IH, Song Y, Lee JA, Halfter W, Balice-Gordon RJ, Linney E, Cole GJ.
|
| タイトル |
Agrin is required for posterior development and motor axon outgrowth and branching in embryonic zebrafish.
|
| ジャーナル |
Glycobiology
|
| Abstract |
Although recent studies have extended our understanding of agrin's function during development, its function in the central nervous system (CNS) is not clearly understood. To address this question, zebrafish agrin was identified and characterized. Zebrafish agrin is expressed in the developing CNS and in nonneural structures such as somites and notochord. In agrin morphant embryos, acetylcholine receptor (AChR) cluster number and size on muscle fibers at the choice point were unaffected, whereas AChR clusters on muscle fibers in the dorsal and ventral regions of the myotome were reduced or absent. Defects in the axon outgrowth by primary motor neurons, subpopulations of branchiomotor neurons, and Rohon-Beard sensory neurons were also observed, which included truncation of axons and increased branching of motor axons. Moreover, agrin morphants exhibit significantly inhibited tail development in a dose-dependent manner, as well as defects in the formation of the midbrain-hindbrain boundary and reduced size of eyes and otic vesicles. Together these results show that agrin plays an important role in both peripheral and CNS development and also modulates posterior development in zebrafish.
|
| 巻・号 |
17(2)
|
| ページ |
231-47
|
| 公開日 |
2007-2-1
|
| DOI |
10.1093/glycob/cwl069
|
| PII |
cwl069
|
| PMID |
17110391
|
| MeSH |
Agrin / analysis
Agrin / genetics
Agrin / physiology*
Animals
Axons / chemistry
Axons / physiology
Cell Differentiation
Embryo, Nonmammalian
Embryonic Development / genetics
Motor Neurons / chemistry
Motor Neurons / cytology
Motor Neurons / physiology*
Muscle, Skeletal / embryology
Muscle, Skeletal / innervation
Nervous System / chemistry
Nervous System / embryology*
RNA, Messenger / analysis
RNA, Messenger / metabolism
Receptors, Cholinergic / analysis
Receptors, Cholinergic / metabolism
Zebrafish / abnormalities
Zebrafish / embryology*
Zebrafish Proteins / analysis
Zebrafish Proteins / genetics
Zebrafish Proteins / physiology*
|
| IF |
4.06
|
| 引用数 |
29
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ゼブラフィッシュ |
rw0 |
