RRC ID 2506
Author Nasuda S, Kikkawa Y, Ashida T, Islam AK, Sato K, Endo TR.
Title Chromosomal assignment and deletion mapping of barley EST markers.
Journal Genes Genet. Syst.
Abstract From about 10000 PCR-based EST markers of barley we chose 1421 EST markers that were demonstrated to be amplified differently by PCR between wheat (Triticum aestivum cv. Chinese Spring) and barley (Hordeum vulgare cv. Betzes). We assigned them to the seven barley chromosomes (1H to 7H) by PCR analysis using a set of wheat-barley chromosome addition lines. We successfully assigned 701 (49.3%) EST markers to the barley chromosomes: 75 to 1H, 127 to 2H, 119 to 3H, 94 to 4H, 108 to 5H, 81 to 6H and 97 to 7H. By using a set of Betzes barley telosomic addition lines of Chinese Spring, we could successfully determine the chromosome-arm (S or L) location of at least 90% of the EST markers assigned to each barley chromosome. We conducted a trial mapping using 90 EST markers assigned to 7HS (49) or 7HL (41) and 19 wheat lines carrying 7H structural changes. More EST markers were found in the distal region than in the proximal region.
Volume 80(5)
Pages 357-66
Published 2005-10
PII JST.JSTAGE/ggs/80.357
PMID 16394587
MeSH Chromosome Deletion* Chromosomes, Plant / genetics* Genetic Markers / genetics Hordeum / genetics* In Situ Hybridization, Fluorescence
IF 0.859
Times Cited 47
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Barley EST Clones H602 Akashinriki Haruna Nijo
Wheat LPGKU2085~2104