RRC ID 2506
著者 Nasuda S, Kikkawa Y, Ashida T, Islam AK, Sato K, Endo TR.
タイトル Chromosomal assignment and deletion mapping of barley EST markers.
ジャーナル Genes Genet Syst
Abstract From about 10000 PCR-based EST markers of barley we chose 1421 EST markers that were demonstrated to be amplified differently by PCR between wheat (Triticum aestivum cv. Chinese Spring) and barley (Hordeum vulgare cv. Betzes). We assigned them to the seven barley chromosomes (1H to 7H) by PCR analysis using a set of wheat-barley chromosome addition lines. We successfully assigned 701 (49.3%) EST markers to the barley chromosomes: 75 to 1H, 127 to 2H, 119 to 3H, 94 to 4H, 108 to 5H, 81 to 6H and 97 to 7H. By using a set of Betzes barley telosomic addition lines of Chinese Spring, we could successfully determine the chromosome-arm (S or L) location of at least 90% of the EST markers assigned to each barley chromosome. We conducted a trial mapping using 90 EST markers assigned to 7HS (49) or 7HL (41) and 19 wheat lines carrying 7H structural changes. More EST markers were found in the distal region than in the proximal region.
巻・号 80(5)
ページ 357-66
公開日 2005-10-1
DOI 10.1266/ggs.80.357
PII JST.JSTAGE/ggs/80.357
PMID 16394587
MeSH Chromosome Deletion* Chromosomes, Plant / genetics* Genetic Markers / genetics Hordeum / genetics* In Situ Hybridization, Fluorescence
IF 0.917
引用数 47
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
オオムギ EST Clones H602 Akashinriki Haruna Nijo
コムギ LPGKU2085~2104