| RRC ID |
2545
|
| 著者 |
Alcântara-Neves NM, Ribeiro-dos-Santos R, Amor AL, Uemura H, Silva-Neto SJ, Eichinger D, Pontes-de-Carvalho L.
|
| タイトル |
Parasite-derived trans-sialidase binds to heart tissue in Trypanosoma cruzi-infected animals.
|
| ジャーナル |
Microb Pathog
|
| Abstract |
Trypanosoma cruzi is an obligate intracellular protozoan parasite that actively penetrates into non-phagocytic mammalian cells. To accomplish this, the parasite relies on the binding of cell surface ligands. It is reported herein that the T. cruzi trans-sialidase (TS), which is exposed on the parasite surface, binds to mouse heart cells, and should therefore be further studied as a possible cell penetration-related ligand. In addition, as has been proposed elsewhere, the binding of T. cruzi to tissues may turn them into targets for parasite-specific immune reactions. Washed heart sections from T. cruzi-infected mice were subjected to immunoenzymatic staining with antisera against whole T. cruzi and with polyclonal or monoclonal antibodies against TS. The anti-TS antibodies stained both parasites and uninfected heart cells in the vicinity of T. cruzi nest remains/trypomastigotes. On the other hand, an anti-T. cruzi serum, which did not recognize TS, only stained the parasites. In addition, normal heart sections from uninfected nude mice were shown to react with both enzymatically active and inactive recombinant TS molecules, probably through their amino-terminal region, since a recombinant TS lacking this region failed to bind.
|
| 巻・号 |
37(5)
|
| ページ |
273-8
|
| 公開日 |
2004-11-1
|
| DOI |
10.1016/j.micpath.2004.03.003
|
| PII |
S0882-4010(04)00100-7
|
| PMID |
15519048
|
| MeSH |
Animals
Antibodies, Protozoan / blood
Glycoproteins
In Vitro Techniques
Mice
Mice, Inbred BALB C
Mice, Nude
Myocardium / metabolism*
Myocytes, Cardiac / cytology
Myocytes, Cardiac / metabolism*
Myocytes, Cardiac / parasitology
Neuraminidase / metabolism*
Trypanosoma cruzi / enzymology*
Trypanosoma cruzi / immunology
Virulence
|
| IF |
2.914
|
| 引用数 |
3
|
|
WOS 分野
|
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
T. cruzi? |
