| RRC ID |
2547
|
| 著者 |
Elshahed MS, Savage KN, Oren A, Gutierrez MC, Ventosa A, Krumholz LR.
|
| タイトル |
Haloferax sulfurifontis sp. nov., a halophilic archaeon isolated from a sulfide- and sulfur-rich spring.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A pleomorphic, extremely halophilic archaeon (strain M6(T)) was isolated from a sulfide- and sulfur-rich spring in south-western Oklahoma (USA). It formed small (0.8-1.0 mm), salmon pink, elevated colonies on agar medium. The strain grew in a wide range of NaCl concentrations (6 % to saturation) and required at least 1 mM Mg(2+) for growth. Strain M6(T) was able to reduce sulfur to sulfide anaerobically. 16S rRNA gene sequence analysis indicated that strain M6(T) belongs to the family Halobacteriaceae, genus Haloferax; it showed 96.7-98.0 % similarity to other members of the genus with validly published names and 89 % similarity to Halogeometricum borinquense, its closest relative outside the genus Haloferax. Polar lipid analysis and DNA G+C content further supported placement of strain M6(T) in the genus Haloferax. DNA-DNA hybridization values, as well as biochemical and physiological characterization, allowed strain M6(T) to be differentiated from other members of the genus Haloferax. A novel species, Haloferax sulfurifontis sp. nov., is therefore proposed to accommodate the strain. The type strain is M6(T) (=JCM 12327(T)=CCM 7217(T)=DSM 16227(T)=CIP 108334(T)).
|
| 巻・号 |
54(Pt 6)
|
| ページ |
2275-2279
|
| 公開日 |
2004-11-1
|
| DOI |
10.1099/ijs.0.63211-0
|
| PMID |
15545470
|
| MeSH |
Anaerobiosis
Base Composition
DNA, Archaeal / chemistry
DNA, Archaeal / isolation & purification
DNA, Ribosomal / chemistry
DNA, Ribosomal / isolation & purification
Fresh Water / microbiology*
Genes, rRNA
Haloferax / classification*
Haloferax / genetics
Haloferax / isolation & purification*
Haloferax / physiology
Lipids / analysis
Lipids / isolation & purification
Magnesium Chloride / metabolism
Molecular Sequence Data
Nucleic Acid Hybridization
Oklahoma
Oxidation-Reduction
Phylogeny
Pigments, Biological / biosynthesis
RNA, Archaeal / genetics
RNA, Ribosomal, 16S / genetics
Saline Solution, Hypertonic
Sequence Analysis, DNA
Sulfides / metabolism
Sulfur / metabolism
Water Microbiology*
|
| IF |
2.415
|
| 引用数 |
27
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
JCM 12327? |
