| RRC ID |
2563
|
| 著者 |
Lan PT, Sakamoto M, Benno Y.
|
| タイトル |
Effects of two probiotic Lactobacillus strains on jejunal and cecal microbiota of broiler chicken under acute heat stress condition as revealed by molecular analysis of 16S rRNA genes.
|
| ジャーナル |
Microbiol Immunol
|
| Abstract |
We examined the effects of probiotic Lactobacillus strains of Lactobacillus agilis JCM 1048 and Lactobacillus salivarius subsp. salicinius JCM 1230 on jejunal and cecal microbiota of broiler chicken under heat stress condition using terminal restriction fragment length polymorphism (T-RFLP) analysis. The jejunal bacterial community was limited to a few bacterial groups, mostly Lactobacillus spp. A relatively abundant and higher prevalence of Lactobacillus spp. were observed in the jejunal and cecal microbiota of the probiotic chickens compared with those of the control chickens under heat stress condition. In general, the probiotic strains did not significantly affect the abundance of L. agilis and L. salivarius in chicken intestine but clearly contributed to increasing their prevalence in the probiotic chickens. The probiotic Lactobacillus strains enriched the diversity of Lactobacillus flora in chicken jejunum and cecum by increasing the abundance and prevalence of Lactobacillus spp. inhabiting the intestine. The richness of Lactobacillus species tended to be similar among the jejunal and cecal microbiota. The bacterial community of cecum was complex and age-dependent. The major components of the cecal microbiota were clostridia and lactobacilli. The Clostridium subcluster XIVa was the most predominant group in chicken cecum. Probiotic Lactobacillus strains restored the microbial balance and maintained the natural stability of indigenous bacterial microbiota following heat stress-induced changes.
|
| 巻・号 |
48(12)
|
| ページ |
917-29
|
| 公開日 |
2004-1-1
|
| DOI |
10.1111/j.1348-0421.2004.tb03620.x
|
| PII |
JST.JSTAGE/mandi/48.917
|
| PMID |
15611608
|
| MeSH |
Age Factors
Animals
Cecum / microbiology*
Chickens / microbiology*
Cluster Analysis
Colony Count, Microbial / veterinary
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
Heat Stress Disorders / microbiology*
Jejunum / microbiology*
Lactobacillus / genetics
Lactobacillus / physiology*
Polymerase Chain Reaction / veterinary
Polymorphism, Restriction Fragment Length
Probiotics / pharmacology*
RNA, Ribosomal, 16S / analysis*
RNA, Ribosomal, 16S / chemistry
RNA, Ribosomal, 16S / genetics
Random Allocation
Statistics, Nonparametric
|
| IF |
1.566
|
| 引用数 |
71
|
|
WOS 分野
|
IMMUNOLOGY
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
JCM 1048?
JCM 1230? |
