RRC ID 2564
著者 Lau SCK, Tsoi MMY, Li X, Plakhotnikova I, Wu M, Wong PK, Qian PY.
タイトル Loktanella hongkongensis sp. nov., a novel member of the alpha-Proteobacteria originating from marine biofilms in Hong Kong waters.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-negative, non-motile, non-spore-forming, short rod-shaped bacterium (UST950701-009P(T)) was isolated from a marine biofilm in Hong Kong waters. Colonies are pink in colour, convex with a smooth surface and entire edge. Brown diffusible pigment is produced. Whitish colonies, with otherwise identical morphology, emerge from every culture upon ageing. The white colonies can be maintained as separate cultures (UST950701-009W) without turning pink. UST950701-009P(T) and UST950701-009W have identical 16S rRNA gene sequences and similar G+C (65.9-66.2 mol%) and fatty acid (86.22-88.52 % 18 : 1omega7c) contents. Phylogenetic analysis of the 16S rRNA gene sequence places UST950701-009P(T) within the Rhodobacter group of the alpha-subclass of the Proteobacteria. The nearest neighbours belong to the genus Loktanella, with similarity values ranging from 94.5 to 95.5 %. Data on G+C and fatty acid contents support the affiliation to the genus Loktanella. UST950701-009P(T) and -009W are heterotrophic, strictly aerobic and require NaCl for growth (2.0-14.0 %). Both grow in pH 5.0-10.0 and at 8-44 degrees C. Both are positive in oxidase, catalase and beta-galactosidase tests, but they differ in the pattern of carbohydrate oxidation and assimilation. Molecular evidence together with phenotypic characteristics shows that UST950701-009P(T) constitutes a novel species within the genus Loktanella. The name Loktanella hongkongensis sp. nov. is proposed; the type strain is UST950701-009P(T) (=NRRL B-41039(T)=JCM 12479(T)) and a morphovar is UST950701-009W (=NRRL B-41040=JCM 12480).
巻・号 54(Pt 6)
ページ 2281-2284
公開日 2004-11-1
DOI 10.1099/ijs.0.63294-0
PMID 15545471
MeSH Aerobiosis Bacterial Typing Techniques Base Composition Biofilms* Carbohydrate Metabolism Catalase / analysis DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Fatty Acids / analysis Fatty Acids / isolation & purification Genes, rRNA Gentian Violet Hong Kong Hydrogen-Ion Concentration Molecular Sequence Data Movement Oxidoreductases / analysis Phenazines Phylogeny Pigments, Biological / biosynthesis RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Rhodobacteraceae / classification* Rhodobacteraceae / cytology Rhodobacteraceae / isolation & purification* Rhodobacteraceae / physiology Saline Solution, Hypertonic Seawater / microbiology* Sequence Analysis, DNA Spores, Bacterial / cytology Temperature Water Microbiology beta-Galactosidase / analysis
IF 2.415
引用数 42
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 JCM 12479? JCM 12480? JCM 7684?