RRC ID 2565
著者 Li X, Zhang D, Chen F, Ma J, Dong Y, Zhang L.
タイトル Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium.
ジャーナル Int J Syst Evol Microbiol
Abstract Cells of strain LX3(T), isolated from soil, were Gram-negative, facultatively anaerobic, non-motile, capsulated and non-endospore-forming straight rods, able to grow at 10 degrees C, unable to produce gas from lactose at 45 degrees C and unable to produce indole. The isolate converted sucrose to isomaltulose and did not produce detectable glucose by-products. The G+C content of the DNA was 56.4 mol%. Furthermore, comparison of 16S rRNA and rpoB gene sequences showed that the isolate clearly belongs to the genus Klebsiella. The closest phylogenetic relative was Klebsiella pneumoniae, there being 99.3 and 97.5 % similarity in 16S rRNA and rpoB gene sequences, respectively. DNA-DNA hybridization analysis demonstrated a very low level of relatedness to other members of the genus Klebsiella, indicating that the isolated strain and other species in the genus Klebsiella were not related at the species level. The isolate could be differentiated from other previously described members of the genus Klebsiella on the basis of phenotypic differences and 16S rRNA and rpoB gene sequence divergence, together with DNA-DNA reassociation data. Therefore, it is proposed that strain LX3(T) (=DSM 16265(T)=JCM 12419(T)) should be classified as the type strain of a novel species of genus Klebsiella, Klebsiella singaporensis sp. nov.
巻・号 54(Pt 6)
ページ 2131-2136
公開日 2004-11-1
DOI 10.1099/ijs.0.02690-0
PMID 15545446
MeSH Anaerobiosis Bacterial Capsules / biosynthesis Base Composition DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification DNA-Directed RNA Polymerases / genetics Genes, rRNA Gentian Violet Glucose / metabolism Indoles / metabolism Isomaltose / analogs & derivatives* Isomaltose / biosynthesis* Klebsiella / classification* Klebsiella / cytology Klebsiella / isolation & purification* Klebsiella / physiology Lactose / metabolism Molecular Sequence Data Movement Nucleic Acid Hybridization Phenazines Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* Spores, Bacterial / cytology Sucrose / metabolism Temperature
IF 2.415
引用数 50
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 JCM 12419?