RRC ID 25682
Author Kurahashi Y, Terashima A, Takumi S.
Title Variation in dehydration tolerance, ABA sensitivity and related gene expression patterns in D-genome progenitor and synthetic hexaploid wheat lines.
Journal Int J Mol Sci
Abstract The wild wheat Aegilops tauschii Coss. has extensive natural variation available for breeding of common wheat. Drought stress tolerance is closely related to abscisic acid (ABA) sensitivity. In this study, 17 synthetic hexaploid wheat lines, produced by crossing the tetraploid wheat cultivar Langdon with 17 accessions of Ae. tauschii, were used for comparative analysis of natural variation in drought tolerance and ABA sensitivity. Ae. tauschii showed wide natural variation, with weak association between the traits. Drought-sensitive accessions of Ae. tauschii exhibited significantly less ABA sensitivity. D-genome variations observed at the diploid genome level were not necessarily reflected in synthetic wheats. However, synthetic wheats derived from the parental Ae. tauschii accessions with high drought tolerance were significantly more tolerant to drought stress than those from drought-sensitive accessions. Moreover, synthetic wheats with high drought tolerance showed significantly higher ABA sensitivity than drought-sensitive synthetic lines. In the hexaploid genetic background, therefore, weak association of ABA sensitivity with drought tolerance was observed. To study differences in gene expression patterns between stress-tolerant and -sensitive lines, levels of two Cor/Lea and three transcription factor gene transcripts were compared. The more tolerant accession of Ae. tauschii tended to accumulate more abundant transcripts of the examined genes than the sensitive accession under stress conditions. The expression patterns in the synthetic wheats seemed to be additive for parental lines exposed to drought and ABA treatments. However, the transcript levels of transcription factor genes in the synthetic wheats did not necessarily correspond to the postulated levels based on expression in parental lines. Allopolyploidization altered the expression levels of the stress-responsive genes in synthetic wheats.
Volume 10(6)
Pages 2733-2751
Published 2009-6-18
DOI 10.3390/ijms10062733
PII i10062733
PMID 19582226
PMC PMC2705513
MeSH Abscisic Acid / pharmacology* Droughts Genome* Plant Leaves / metabolism Plant Proteins / genetics Plant Proteins / metabolism RNA, Plant / metabolism Transcription Factors / genetics Transcription Factors / metabolism Transcriptome / drug effects Triticum / drug effects* Triticum / growth & development Triticum / metabolism
IF 4.556
Times Cited 26
Wheat KU-2059 KU-2027 KU-2811 KU-2816 KU-20-1 KU-2829A KU-2076 KU-2078 KU-2093 KU-2126 KU-2160 KU-2069 KU-2088