| RRC ID |
25686
|
| Author |
Andoh T, Takayama Y, Yamakoshi T, Lee JB, Sano A, Shimizu T, Kuraishi Y.
|
| Title |
Involvement of serine protease and proteinase-activated receptor 2 in dermatophyte-associated itch in mice.
|
| Journal |
J Pharmacol Exp Ther
|
| Abstract |
We investigated the involvement of serine protease and proteinase-activated receptor 2 (PAR(2)) in dermatophyte-induced itch in mice. An intradermal injection of an extract of the dermatophyte Arthroderma vanbreuseghemii (ADV) induced hind-paw scratching, an itch-related behavior. ADV extract-induced scratching was inhibited by the opioid receptor antagonists naloxone and naltrexone, the serine protease inhibitor nafamostat mesylate, and the PAR(2) receptor antagonist FSLLRY-NH(2). ADV extract-induced scratching was not inhibited by the H(1) histamine receptor antagonist terfenadine or by mast cell deficiency. Heat pretreatment of the ADV extract markedly reduced the scratch-inducing and serine protease activities. Proteolytic cleavage within the extracellular N terminus of the PAR(2) receptor exposes a sequence that serves as a tethered ligand for the receptor. The ADV extract as well as tryptase and trypsin cleaved a synthetic N-terminal peptide of the PAR(2) receptor. The present results suggest that serine protease secreted by dermatophytes causes itching through activation of the PAR(2) receptors, which may be a causal mechanism of dernatophytosis itch.
|
| Volume |
343(1)
|
| Pages |
91-6
|
| Published |
2012-10-1
|
| DOI |
10.1124/jpet.112.195222
|
| PII |
jpet.112.195222
|
| PMID |
22761302
|
| MeSH |
Animals
Arthrodermataceae*
Male
Mice
Mice, Inbred ICR
Mice, Knockout
Oligopeptides / pharmacology
Pruritus / enzymology*
Receptor, PAR-2 / antagonists & inhibitors
Receptor, PAR-2 / physiology*
Serine Proteases / physiology*
|
| IF |
3.561
|
| Times Cited |
15
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
|
| Resource |
| INFORMATION |
nbrp |
