| RRC ID |
2594
|
| Author |
Takai K, Nealson KH, Horikoshi K.
|
| Title |
Methanotorris formicicus sp. nov., a novel extremely thermophilic, methane-producing archaeon isolated from a black smoker chimney in the Central Indian Ridge.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A novel extremely thermophilic, methane-producing archaeon was isolated from a black smoker chimney at the Kairei field in the Central Indian Ridge. Cells of this isolate were irregular cocci with several flagella; motility was not observed. Growth was observed between 55 and 83 degrees C (optimum of 75 degrees C; 30 min doubling time) and between pH 6.0 and 8.5 (optimum of pH 6.7). The isolate was a strictly anaerobic, methanogenic autotroph capable of using hydrogen and carbon dioxide as sole energy and carbon sources. Formate was utilized as an alternative energy source. The G+C content of the genomic DNA was 33.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was most closely related to Methanotorris igneus strain Kol 5T. The isolate, however, could be genetically differentiated from this species by DNA-DNA hybridization analysis and on the basis of its physiological properties. The name Methanotorris formicicus sp. nov. is proposed for this isolate; the type strain is Mc-S-70T (=JCM 11930T=ATCC BAA-687T).
|
| Volume |
54(Pt 4)
|
| Pages |
1095-1100
|
| Published |
2004-7-1
|
| DOI |
10.1099/ijs.0.02887-0
|
| PMID |
15280275
|
| MeSH |
Anaerobiosis
Base Composition
Carbon Dioxide / metabolism
DNA, Bacterial / chemistry
DNA, Bacterial / isolation & purification
DNA, Ribosomal / chemistry
Flagella / ultrastructure
Formates / metabolism
Genes, rRNA
Hydrogen / metabolism
Hydrogen-Ion Concentration
Indian Ocean
Methane / metabolism*
Methanococcales / classification*
Methanococcales / cytology
Methanococcales / growth & development
Methanococcales / isolation & purification*
Molecular Sequence Data
Movement
Nucleic Acid Hybridization
Phylogeny
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sequence Homology
Temperature
Water Microbiology
|
| IF |
2.415
|
| Times Cited |
29
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Pathogenic microorganisms |
JCM 11930?
JCM 10045?
JCM 10549?
JCM 10722? |
