RRC ID 2617
著者 Yumoto I, Hirota K, Kawahara T, Nodasaka Y, Okuyama H, Matsuyama H, Yokota Y, Nakajima K, Hoshino T.
タイトル Anoxybacillus voinovskiensis sp. nov., a moderately thermophilic bacterium from a hot spring in Kamchatka.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel moderately thermophilic bacterium, strain TH13T, was isolated from a hot spring in Kamchatka. It was found to be a Gram-positive, facultative aerobe; the straight, non-motile rods grew at 30-64 degrees C (optimum 54 degrees C). The isolate was positive for catalase and oxidase tests and reduced nitrate to nitrite, but was negative for H2S production and growth in more than 3% NaCl (w/v). The isolate grew at pH 7-8, but not at pH values higher than 9. The DNA G+C content was 43.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain TH13T was a member of the genus Anoxybacillus. DNA-DNA hybridization revealed a low relatedness (less than 30.2%) between the isolate and its close phylogenetic neighbours Anoxybacillus pushchinoensis and Anoxybacillus flavithermus. On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA hybridization data, it was concluded that the isolate merited classification as a novel species, for which the name Anoxybacillus voinovskiensis sp. nov. is proposed. The type strain of this species is TH13T (=NCIMB 13956T=JCM 12111T).
巻・号 54(Pt 4)
ページ 1239-1242
公開日 2004-7-1
DOI 10.1099/ijs.0.02889-0
PMID 15280298
MeSH Aerobiosis Anaerobiosis Bacillaceae / classification* Bacillaceae / cytology Bacillaceae / isolation & purification* Bacillaceae / physiology Bacterial Typing Techniques Base Composition Catalase / metabolism DNA, Bacterial / chemistry DNA, Ribosomal / chemistry Genes, rRNA Gentian Violet Growth Inhibitors / pharmacology Hot Springs / microbiology* Hydrogen Sulfide / metabolism Hydrogen-Ion Concentration Molecular Sequence Data Movement Nitrates / metabolism Nucleic Acid Hybridization Oxidation-Reduction Oxidoreductases / metabolism Phenazines Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Russia Sequence Analysis, DNA Sequence Homology Sodium Chloride / pharmacology Temperature Water Microbiology
IF 2.415
引用数 34
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 JCM 12111?