RRC ID |
2617
|
著者 |
Yumoto I, Hirota K, Kawahara T, Nodasaka Y, Okuyama H, Matsuyama H, Yokota Y, Nakajima K, Hoshino T.
|
タイトル |
Anoxybacillus voinovskiensis sp. nov., a moderately thermophilic bacterium from a hot spring in Kamchatka.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
A novel moderately thermophilic bacterium, strain TH13T, was isolated from a hot spring in Kamchatka. It was found to be a Gram-positive, facultative aerobe; the straight, non-motile rods grew at 30-64 degrees C (optimum 54 degrees C). The isolate was positive for catalase and oxidase tests and reduced nitrate to nitrite, but was negative for H2S production and growth in more than 3% NaCl (w/v). The isolate grew at pH 7-8, but not at pH values higher than 9. The DNA G+C content was 43.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain TH13T was a member of the genus Anoxybacillus. DNA-DNA hybridization revealed a low relatedness (less than 30.2%) between the isolate and its close phylogenetic neighbours Anoxybacillus pushchinoensis and Anoxybacillus flavithermus. On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA hybridization data, it was concluded that the isolate merited classification as a novel species, for which the name Anoxybacillus voinovskiensis sp. nov. is proposed. The type strain of this species is TH13T (=NCIMB 13956T=JCM 12111T).
|
巻・号 |
54(Pt 4)
|
ページ |
1239-1242
|
公開日 |
2004-7-1
|
DOI |
10.1099/ijs.0.02889-0
|
PMID |
15280298
|
MeSH |
Aerobiosis
Anaerobiosis
Bacillaceae / classification*
Bacillaceae / cytology
Bacillaceae / isolation & purification*
Bacillaceae / physiology
Bacterial Typing Techniques
Base Composition
Catalase / metabolism
DNA, Bacterial / chemistry
DNA, Ribosomal / chemistry
Genes, rRNA
Gentian Violet
Growth Inhibitors / pharmacology
Hot Springs / microbiology*
Hydrogen Sulfide / metabolism
Hydrogen-Ion Concentration
Molecular Sequence Data
Movement
Nitrates / metabolism
Nucleic Acid Hybridization
Oxidation-Reduction
Oxidoreductases / metabolism
Phenazines
Phylogeny
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Russia
Sequence Analysis, DNA
Sequence Homology
Sodium Chloride / pharmacology
Temperature
Water Microbiology
|
IF |
2.415
|
引用数 |
34
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
病原微生物 |
JCM 12111? |