RRC ID 2619
Author Yumoto I, Hirota K, Yamaga S, Nodasaka Y, Kawasaki T, Matsuyama H, Nakajima K.
Title Bacillus asahii sp. nov., a novel bacterium isolated from soil with the ability to deodorize the bad smell generated from short-chain fatty acids.
Journal Int J Syst Evol Microbiol
Abstract In a screening campaign to isolate strains with the ability to remove the bad smell associated with animal faeces, strain MA001(T) was isolated from a soil sample obtained from Shizuoka prefecture, Japan. The isolate grew at pH 6-9 but not at pH 10. Cells were Gram-positive, straight rods with peritrichous flagella and produced ellipsoidal spores. The isolate was positive for catalase and oxidase tests but negative for indole production, deamination of phenylalanine and H(2)S production. The isolate did not produce acid from any carbohydrates tested and could not grow in more than 2 % NaCl. The DNA G+C content was 39.4 mol%. The cellular fatty acids profile consisted of significant amount of C(15) branched-chain fatty acids, iso-C(15 : 0) and anteiso-C(15 : 0). Phylogenetic analysis based on 16S rRNA gene sequencing indicated that strain MA001(T) was closely related to Bacillus simplex and Bacillus psychrosaccharolyticus. DNA-DNA hybridization revealed a low relatedness of the isolate to several phylogenetically close neighbours (less than 9 %). On the basis of the phenotypic characteristics observed, phylogenetic data based on 16S rRNA gene sequencing and DNA-DNA relatedness data, it is concluded that the isolate should be classified as representing a novel species, for which the name Bacillus asahii is proposed. The type strain is MA001(T) (=JCM 12112(T)=NCIMB 13969(T)).
Volume 54(Pt 6)
Pages 1997-2001
Published 2004-11-1
DOI 10.1099/ijs.0.03014-0
PMID 15545424
MeSH Amino Acid Oxidoreductases / analysis Bacillus / classification* Bacillus / cytology Bacillus / isolation & purification* Bacillus / physiology Bacterial Typing Techniques Base Composition Carbohydrate Metabolism Carboxylic Acids / metabolism Catalase / analysis DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Fatty Acids / analysis Fatty Acids / isolation & purification Fatty Acids, Volatile / metabolism* Flagella Genes, rRNA Gentian Violet Hydrogen Sulfide / metabolism Hydrogen-Ion Concentration Indoles / metabolism Japan Molecular Sequence Data Nucleic Acid Hybridization Odorants* Oxidoreductases / analysis Phenazines Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Saline Solution, Hypertonic Sequence Analysis, DNA Soil Microbiology* Spores, Bacterial / cytology
IF 2.415
Times Cited 38
Pathogenic microorganisms JCM 12112?