RRC ID 2620
著者 Yumoto I, Hishinuma-Narisawa M, Hirota K, Shingyo T, Takebe F, Nodasaka Y, Matsuyama H, Hara I.
タイトル Exiguobacterium oxidotolerans sp. nov., a novel alkaliphile exhibiting high catalase activity.
ジャーナル Int J Syst Evol Microbiol
Abstract A novel alkaliphile was isolated from a drain of a fish processing plant. The isolate grew at a pH range of 7-10. Cells were Gram-positive, facultatively aerobic, motile rods with peritrichous flagella. Colonies were orange or yellow in colour. Catalase and oxidase reactions were positive. The isolate grew in 0-12 % NaCl but not above 15 % NaCl. Its cell extract exhibited 567 times higher catalase activity than an Escherichia coli cell extract. The major cellular fatty acids were iso-C(13 : 0), anteiso-C(13 : 0), iso-C(15 : 0), iso-C(16 : 0), iso-C(17 : 0), anteiso-C(17 : 0) and iso-C(17 : 1). Its DNA G+C content was 46.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequencing and chemotaxonomic data indicated that strain T-2-2(T) is a member of the genus Exiguobacterium. DNA-DNA hybridization revealed a low relatedness of the isolate to several phylogenetic neighbours (less than 25 %). On the basis of phenotypic characteristics, phylogenetic data and DNA-DNA relatedness data, the isolate merits classification as a novel species, for which the name Exiguobacterium oxidotolerans sp. nov. is proposed. The type strain is T-2-2(T) (=JCM 12280(T)=NCIMB 13980(T)).
巻・号 54(Pt 6)
ページ 2013-2017
公開日 2004-11-1
DOI 10.1099/ijs.0.63129-0
PMID 15545426
MeSH Aerobiosis Bacillaceae / classification* Bacillaceae / cytology Bacillaceae / enzymology Bacillaceae / isolation & purification* Bacterial Typing Techniques Base Composition Catalase / metabolism* DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Escherichia coli / metabolism Fatty Acids / analysis Fatty Acids / isolation & purification Flagella Genes, rRNA Gentian Violet Hydrogen-Ion Concentration Molecular Sequence Data Movement Nucleic Acid Hybridization Oxidoreductases / analysis Phenazines Phylogeny Pigments, Biological / biosynthesis RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Saline Solution, Hypertonic Sequence Analysis, DNA Sewage / microbiology* Water Microbiology*
IF 2.415
引用数 48
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 JCM 12280? JCM 1968? JCM 1464? JCM 1027? JCM 5963? JCM 2147?