RRC ID 26209
Author Tan S, Ho KK.
Title Purification of an acidic plastocyanin from Microcystis aeruginosa.
Journal Biochim Biophys Acta
Abstract Plastocyanin and cytochrome c-553 are two functionally equivalent electron carriers in the photosynthetic chain of cyanobacteria. Microcystis aeruginosa, a unicellular cyanobacterium which grows well at a high pH (8.6) and which was not known to possess plastocyanin, has been studied for its ability to synthesize plastocyanin in culture media with and without Cu. In the absence of Cu, an acidic cytochrome c-553 alone was isolated. With the inclusion of 2 microM Cu, cytochrome c-553 synthesis was partially suppressed and an acidic plastocyanin was isolated. A newly developed procedure, using high concentrations of ammonium sulfate to fractionate water-soluble proteins on Sephacryl S-200 was successfully used to isolate and concentrate the plastocyanin, thus allowing it to be further purified to homogeneity. This protein has an isoelectric point of 4.8 which is similar to the pI value reported for other acidic plastocyanins from higher plants and green algae. Its N-terminal sequence of the first 15 amino acids has been determined; 9 of these amino acids are identical to those in the sequence of the basic plastocyanin from Anabaena variabilis.
Volume 973(2)
Pages 111-7
Published 1989-2-28
DOI 10.1016/s0005-2728(89)80410-4
PII S0005-2728(89)80410-4
PMID 2537099
MeSH Amino Acid Sequence Ammonium Sulfate Copper / pharmacology Cytochrome c Group / biosynthesis Cytochrome c Group / isolation & purification Fractional Precipitation Isoelectric Point Microcystis / analysis* Microcystis / drug effects Microcystis / metabolism Molecular Sequence Data Molecular Weight Plant Proteins / isolation & purification* Plastocyanin / isolation & purification* Protein Denaturation Spectrophotometry
IF 3.411
Times Cited 7
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Algae NIES-44