RRC ID 2623
著者 Bae SS, Lee JH, Kim SJ.
タイトル Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough.
ジャーナル Int J Syst Evol Microbiol
Abstract Two thermophilic, spore-forming strains, TM1(T) and TM5, were isolated from deep-sea sediment (4000 m below sea level) of the Ayu Trough in the western Pacific Ocean. Cells of the two strains were Gram-positive, motile and rod-shaped. Their spores were ellipsoidal, subterminal to terminal and occurred in swollen sporangia. The two strains grew at temperatures up to 65 degrees C and in the pH range 6.5-9.0. The NaCl concentration for optimal growth was 3.0 % (w/v) and growth was inhibited by 5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains TM1(T) and TM5 belonged to the genus Bacillus, and that strain TM1(T) was most closely related to Bacillus aeolius DSM 15084(T) (96.7 %), Bacillus smithii DSM 4216(T) (96.1 %), Bacillus methanolicus NCIMB 13113(T) (95.8 %) and Bacillus pallidus DSM 3670(T) (95.7 %). Between the 16S rRNA gene sequences of strains TM1(T) and TM5 there were only three nucleotide differences, implying that the two strains were of the same species. The cellular fatty acid profiles of the two strains were also very similar, with iso-C(15 : 0), iso-C(16 : 0), C(16 : 0), iso-C(17 : 0) and anteiso-C(17 : 0) as the major components. The G + C content of strain TM1(T) was 38.7 %. On the basis of phenotypic and molecular data, strains TM1(T) and TM5 represent a novel species of the genus Bacillus, for which the name Bacillus alveayuensis sp. nov. is proposed. The type strain is TM1(T) (= KCTC 10634(T) = JCM 12523(T)).
巻・号 55(Pt 3)
ページ 1211-1215
公開日 2005-5-1
DOI 10.1099/ijs.0.63424-0
PMID 15879257
MeSH Bacillus / classification* Bacillus / cytology Bacillus / isolation & purification* Bacillus / physiology Bacterial Typing Techniques Base Composition DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Fatty Acids / analysis Fatty Acids / isolation & purification Genes, rRNA Gentian Violet Geologic Sediments / microbiology* Growth Inhibitors / pharmacology Hot Temperature Molecular Sequence Data Movement Pacific Ocean Phenazines Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / pharmacology Spores, Bacterial / cytology Water Microbiology
IF 2.415
引用数 47
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 JCM 12523?