| RRC ID |
2649
|
| 著者 |
Iida T, Nakamura K, Izumi A, Mukouzaka Y, Kudo T.
|
| タイトル |
Isolation and characterization of a gene cluster for dibenzofuran degradation in a new dibenzofuran-utilizing bacterium, Paenibacillus sp. strain YK5.
|
| ジャーナル |
Arch Microbiol
|
| Abstract |
Spore-forming bacterial strains capable of utilizing dibenzofuran (DF) as a sole source of carbon and energy were isolated. Characteristics of the isolates justified their classification into the genus Paenibacillus, and their closest relative was P. naphthalenovorans. Degenerate primers for aromatic hydrocarbon dioxygenase alpha subunit (AhDOa) genes and genomic DNA of the strain YK5 were used for gene isolation. The nucleotide sequences of clones of the PCR products revealed that the strain YK5 carries at least five different AhDOa genes. Northern hybridization analysis showed that one of the AhDOa genes was transcribed under DF-containing culture conditions. A gene cluster encoding the AhDOa was isolated. The genes predicted to encode extradiol dioxygenase (dbfB) and hydrolase (dbfC) were found to be an upstream of genes encoding the alpha and beta subunit of the AhDO (dbfA1 and dbfA2, respectively); the latter two gene products showed 60 and 53% identity to the amino acid sequences of DbfA1 and DbfA2 of Terrabacter sp. DBF63, respectively. Two Paenibacillus validus JCM 9077 strains transformed with the dbf gene clusters acquired the ability to convert DF to 2,2',3-trihydroxybiphenyl (THBP) and salicylic acid (SAL). These results suggest that the enzymes encoded by the gene cluster isolated in this study are involved in DF metabolism in YK5.
|
| 巻・号 |
184(5)
|
| ページ |
305-15
|
| 公開日 |
2006-1-1
|
| DOI |
10.1007/s00203-005-0045-9
|
| PMID |
16284749
|
| MeSH |
Base Composition
Benzofurans / metabolism*
Cloning, Molecular
Dioxygenases / genetics
Dioxygenases / metabolism
Gram-Positive Bacteria / classification
Gram-Positive Bacteria / enzymology*
Gram-Positive Bacteria / genetics
Gram-Positive Bacteria / physiology
Hydrocarbons, Aromatic / metabolism
Hydrolases / genetics
Hydrolases / metabolism
Molecular Sequence Data
Multigene Family*
Oxygenases / genetics
Oxygenases / metabolism
Sequence Analysis, DNA
Spores, Bacterial / physiology
|
| IF |
1.884
|
| 引用数 |
18
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
JCM 9077? |
