| RRC ID |
2699
|
| 著者 |
Postec A, Breton CL, Fardeau ML, Lesongeur F, Pignet P, Querellou J, Ollivier B, Godfroy A.
|
| タイトル |
Marinitoga hydrogenitolerans sp. nov., a novel member of the order Thermotogales isolated from a black smoker chimney on the Mid-Atlantic Ridge.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
A novel, thermophilic, anaerobic bacterium that is able to tolerate hydrogen was isolated from a deep-sea hydrothermal chimney collected at the Rainbow field on the Mid-Atlantic Ridge. Cells were rod-shaped and surrounded by a sheath-like outer structure (toga); they were weakly motile by means of a polar flagellum. They appeared singly, in pairs or in short chains. They grew at 35-65 degrees C (optimum 60 degrees C), pH 4.5-8.5 (optimum pH 6.0) and 10-65 g sea salts l(-1) (optimum 30-40 g l(-1)). The isolate was organotrophic, and able to grow on various carbohydrates or complex proteinaceous substrates. Growth was not inhibited under 100 % hydrogen or in the presence of 2 % oxygen in the gas phase. The isolate reduces sulfur, although sulfur reduction is not required for growth. The fermentation products identified on glucose were acetate, ethanol, formate, hydrogen and CO(2). The G + C content of the genomic DNA was 28 +/- 1 mol%. Phylogenetic analysis of the 16S rRNA gene placed the strain within the genus Marinitoga, order Thermotogales, in the bacterial domain. On the basis of the 16S rRNA gene sequence comparisons and physiological characteristics, the isolate is considered to represent a novel species, for which the name Marinitoga hydrogenitolerans sp. nov. is proposed. The type strain is AT1271(T) (=DSM 16785(T) = JCM 12826(T)).
|
| 巻・号 |
55(Pt 3)
|
| ページ |
1217-1221
|
| 公開日 |
2005-5-1
|
| DOI |
10.1099/ijs.0.63550-0
|
| PMID |
15879258
|
| MeSH |
Acetic Acid / metabolism
Atlantic Ocean
Base Composition
Carbohydrate Metabolism
Carbon Dioxide / metabolism
DNA, Bacterial / chemistry
DNA, Bacterial / isolation & purification
DNA, Ribosomal / chemistry
DNA, Ribosomal / isolation & purification
Ethanol / metabolism
Flagella / physiology
Formates / metabolism
Genes, rRNA
Gram-Negative Anaerobic Straight, Curved, and Helical Rods / classification*
Gram-Negative Anaerobic Straight, Curved, and Helical Rods / cytology
Gram-Negative Anaerobic Straight, Curved, and Helical Rods / isolation & purification*
Gram-Negative Anaerobic Straight, Curved, and Helical Rods / physiology
Hot Temperature
Hydrogen / metabolism
Hydrogen / toxicity
Hydrogen-Ion Concentration
Molecular Sequence Data
Movement
Oxidation-Reduction
Oxygen / toxicity
Phylogeny
Proteins / metabolism
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sequence Analysis, DNA
Sulfur / metabolism
Water Microbiology
|
| IF |
2.415
|
| 引用数 |
23
|
|
WOS 分野
|
MICROBIOLOGY
|
| リソース情報 |
| 病原微生物 |
JCM 12826? |
