RRC ID 2707
Author Saha P, Mondal AK, Mayilraj S, Krishnamurthi S, Bhattacharya A, Chakrabarti T.
Title Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India.
Journal Int. J. Syst. Evol. Microbiol.
Abstract A polyphasic approach was used to characterize a bacterium, GPTSA 11(T), isolated from a warm spring located in a reserve forest in Assam, India. The cells are Gram-variable, strictly aerobic, sporulating motile rods. The major fatty acids of the strain are C(15 : 0) anteiso (48.42 %), C(16 : 0) iso (11.59 %), C(16 : 1)omega11c (6.16 %), C(15 : 0) iso (6.03 %), C(17 : 0) anteiso (5.68 %) and C(16 : 1)omega7c alcohol (5.01 %). The presence of the fatty acid C(16 : 1)omega7c alcohol distinguishes this strain from other closely related species of the genus Paenibacillus. The strain contains MK-7 as the diagnostic menaquinone. The G+C content of the genomic DNA is 41.2 mol%. Analysis of the 16S rRNA gene sequence (1466 nt) revealed the presence of signature sequences PAEN 515F (5'-GAGTAACTGCTCTCGGAATGACGGTACTTGAGAAGAAAGCCCC-3') and PAEN 862F (5'-TCGATACCCTTGGTGCCGAAGT-3'), which were found in the species of the genus Paenibacillus surveyed by Shida et al. [Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. (1997). Int J Syst Bacteriol 47, 289-298]. The sequence shows closest similarity (95.85 %) to that of Paenibacillus apiarius, followed by Paenibacillus alvei (94.34 %), Paenibacillus cineris (93.87 %), Paenibacillus favisporus (93.80 %), Paenibacillus chibensis (93.47 %) and Paenibacillus azoreducens (93.40 %). Biochemical, physiological, chemotaxonomic and phylogenetic analyses justify placement of the strain in the genus Paenibacillus but not within any existing species. It should, therefore, be considered as representing a novel species, for which the name Paenibacillus assamensis sp. nov. is proposed. The type strain is GPTSA 11T (=MTCC 6934T=JCM 13186T).
Volume 55(Pt 6)
Pages 2577-81
Published 2005-11
DOI 10.1099/ijs.0.63846-0
PII 55/6/2577
PMID 16280530
MeSH DNA, Bacterial / analysis DNA, Ribosomal / analysis Fatty Acids / analysis Gram-Positive Endospore-Forming Rods / classification Gram-Positive Endospore-Forming Rods / cytology Gram-Positive Endospore-Forming Rods / isolation & purification* Gram-Positive Endospore-Forming Rods / physiology Hot Springs / microbiology* India Molecular Sequence Data RNA, Ribosomal, 16S / analysis RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 1.932
Times Cited 47
WOS Category MICROBIOLOGY
Resource
Pathogenic microorganisms JCM 13186? JCM 11200?