| RRC ID |
27167
|
| 著者 |
Izumi Y, Aikawa S, Matsuda F, Hasunuma T, Kondo A.
|
| タイトル |
Aqueous size-exclusion chromatographic method for the quantification of cyanobacterial native glycogen.
|
| ジャーナル |
J Chromatogr B Analyt Technol Biomed Life Sci
|
| Abstract |
Cyanobacterial glycogen has gained interest as a valuable biomass feedstock for biofuel production. However, an ideal method for native glycogen quantification has not been developed. Here, we have proposed a simple methodology that enables the quantitative determination of cyanobacterial glycogen concentration with high repeatability using aqueous size-exclusion chromatography with a differential refractive index detector (SEC/RID). Our SEC/RID system also allows size distributions for native glycogen based on hydrodynamic volumes (Vh), which is proportional to the product of the molecular mass (M) and intrinsic viscosity [η], obtained by universal calibration using linear homopolymers of known M with Mark-Houwink-Sakurada parameters. The universal calibration curve achieved a broad linear range (Vh parameter [η]M=2×10(2)-8×10(8)mLg(-1)) with a high correlation coefficient (R(2)=0.9942), because the developed system is equipped with an OHpak SB-806M HQ aqueous column containing four types of polyhydroxy methacrylate-based particles with different particle and pore sizes. Based on the SEC/RID system, response of molecular size distribution of glycogen in microalgae to the cultivation condition was first observed. Our established SEC/RID method has several advantages over conventional techniques, including the simultaneous quantitative and size distribution analyses of glycogen, and represents a potentially useful tool to elucidate the relationship between structural properties and the roles of glycogen in metabolism.
|
| 巻・号 |
930
|
| ページ |
90-7
|
| 公開日 |
2013-7-1
|
| DOI |
10.1016/j.jchromb.2013.04.037
|
| PII |
S1570-0232(13)00260-2
|
| PMID |
23727872
|
| MeSH |
Chromatography, Gel / methods*
Cyanobacteria / chemistry
Cyanobacteria / metabolism*
Glycogen / analysis*
Glycogen / metabolism
Hydrodynamics
Microalgae
Molecular Weight
Polyhydroxyethyl Methacrylate / chemistry
Reproducibility of Results
Temperature
Water / chemistry
|
| IF |
3.004
|
| 引用数 |
14
|
|
WOS 分野
|
CHEMISTRY, ANALYTICAL
BIOCHEMICAL RESEARCH METHODS
|
| リソース情報 |
| 藻類 |
NIES-39 |
