RRC ID 27171
Author Kamimura S, Inoue K, Ogonuki N, Hirose M, Oikawa M, Yo M, Ohara O, Miyoshi H, Ogura A.
Title Mouse cloning using a drop of peripheral blood.
Journal Biol. Reprod.
Abstract 0.05). Because the use of lymphocyte nuclei inevitably results in the birth of offspring with DNA rearrangements, we applied granulocyte/monocyte cloning to two genetically modified strains and two recombinant inbred strains. Normal-looking offspring were obtained from all four strains tested. The present study clearly indicated that genetic copies of mice could be produced using a drop of peripheral blood from living donors. This strategy will be applied to the rescue of infertile founder animals or a "last-of-line" animal possessing invaluable genetic resources.
Volume 89(2)
Pages 24
Published 2013-8
DOI 10.1095/biolreprod.113.110098
PII biolreprod.113.110098
PMID 23803554
MeSH Animals Cloning, Organism / methods* Embryo Transfer Embryo, Mammalian / cytology* Fibroblasts / cytology Flow Cytometry Mice Nuclear Transfer Techniques*
IF 3.184
Times Cited 6
Mice 129XB6-F(RBRC01005) 129XB6-F(RBRC01005) 129XB6-F(RBRC01005)