| RRC ID |
27177
|
| Author |
Kobayashi N, Noguchi H, Westerman KA, Watanabe T, Matsumura T, Totsugawa T, Fujiwara T, Leboulch P, Tanaka N.
|
| Title |
Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes.
|
| Journal |
Cell Transplant
|
| Abstract |
Current clinical reports have indicated that hepatocyte transplantation (HTX) could be used in patients with liver failure and in children with liver-based metabolic diseases. One of the major limiting factors of HTX is a serious shortage of donor livers for hepatocyte isolation. To address this issue, we immortalized adult human hepatocytes with a retroviral vector SSR#69 expressing the genes of simian virus 40 large T antigen and herpes simplex virus-thymidine kinase simultaneously. One of the resulting clones, NKNT-3, grew steadily in chemically defined serum-free medium without any obvious crisis and showed the gene expression of differentiated liver functions. Under the administration of 5 microM ganciclovir, NKNT-3 cells stopped proliferation and died in in vitro experiments. We have established a tightly regulated immortal human hepatocyte cell line. The cells could allow the need for immediate availability of consistent and functionally uniform cells in sufficient quantity and adequate quality.
|
| Volume |
10(4-5)
|
| Pages |
377-81
|
| Published |
2001-1-1
|
| PMID |
11549057
|
| MeSH |
Antigens, Polyomavirus Transforming / genetics*
Antiviral Agents / pharmacology
Cell Line*
Cell Transplantation / methods
Culture Media, Serum-Free
Ganciclovir / pharmacology
Gene Expression
Gene Transfer Techniques*
Hepatocytes / cytology
Hepatocytes / drug effects
Hepatocytes / physiology*
Hepatocytes / transplantation
Humans
Immunohistochemistry
Liver / physiology
Liver Transplantation
Liver, Artificial
Retroviridae / genetics
Retroviridae / metabolism
Simplexvirus / enzymology*
Thymidine Kinase / genetics*
|
| IF |
3.341
|
| Times Cited |
7
|
|
WOS Category
|
TRANSPLANTATION
MEDICINE, RESEARCH & EXPERIMENTAL
CELL & TISSUE ENGINEERING
|
| Resource |
| DNA material |
AxCALacZ (RDB01745) |
