論文 - 詳細
| RRC ID | 2718 |
|---|---|
| 著者 | Sokolova TG, Kostrikina NA, Chernyh NA, Kolganova TV, Tourova TP, Bonch-Osmolovskaya EA. |
| タイトル | Thermincola carboxydiphila gen. nov., sp. nov., a novel anaerobic, carboxydotrophic, hydrogenogenic bacterium from a hot spring of the Lake Baikal area. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A novel anaerobic, thermophilic, alkalitolerant bacterium, strain 2204(T), was isolated from a hot spring of the Baikal Lake region. The cells of strain 2204(T) were straight rods of variable length, Gram-positive with an S-layer, motile with one to two lateral flagella, and often formed aggregates of 3-15 cells. The isolate was shown to be an obligate anaerobe oxidizing CO and producing equimolar quantities of H(2) and CO(2) according to the equation CO+H(2)O-->CO(2)+H(2). No organic substrates were used as energy sources. For lithotrophic growth on CO, 0.2 g acetate or yeast extract l(-1) was required but did not support growth in the absence of CO. Growth was observed in the temperature range 37-68 degrees C, the optimum being 55 degrees C. The pH range for growth was 6.7-9.5, the optimum pH being 8.0. The generation time under optimal conditions was 1.3 h. The DNA G+C content was 45 mol%. Penicillin, erythromycin, streptomycin, rifampicin, vancomycin and tetracycline completely inhibited both growth and CO utilization by strain 2204(T). Thus, isolate 2204(T) was found to be the first known moderately thermophilic and alkalitolerant H(2)-producing anaerobic carboxydotroph. The novel bacterium fell within the cluster of the family Peptococcaceae within the low-G+C-content Gram-positive bacteria, where it formed a separate branch. On the basis of morphological, physiological and phylogenetic features, strain 2204(T) should be assigned to a novel genus and species, for which the name Thermincola carboxydiphila gen. nov., sp. nov. is proposed. The type strain is strain 2204(T) (=DSM 17129(T)=VKM B-2283(T)=JCM 13258(T)). |
| 巻・号 | 55(Pt 5) |
| ページ | 2069-2073 |
| 公開日 | 2005-9-1 |
| DOI | 10.1099/ijs.0.63299-0 |
| PMID | 16166711 |
| MeSH | Anti-Bacterial Agents / pharmacology Bacteria, Anaerobic / classification Bacteria, Anaerobic / genetics Bacteria, Anaerobic / isolation & purification Bacteria, Anaerobic / physiology Carbon Monoxide / metabolism* DNA, Bacterial / analysis DNA, Ribosomal / analysis Genes, rRNA Hot Springs / microbiology* Hot Temperature Hydrogen / metabolism* Hydrogen-Ion Concentration Microbial Sensitivity Tests / methods Molecular Sequence Data Oxidation-Reduction Peptococcaceae / classification* Peptococcaceae / genetics Peptococcaceae / isolation & purification Peptococcaceae / physiology Phylogeny RNA, Ribosomal, 16S / genetics Russia Species Specificity |
| IF | 2.415 |
| 引用数 | 45 |
| WOS 分野 | MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | JCM 13258? |