論文 - 詳細
| RRC ID | 27202 |
|---|---|
| 著者 | Kamachi Y, Kondoh H. |
| タイトル | Overlapping positive and negative regulatory elements determine lens-specific activity of the delta 1-crystallin enhancer. |
| ジャーナル | Mol Cell Biol |
| Abstract |
Lens-specific expression of the delta 1-crystallin gene is governed by an enhancer in the third intron, and the 30-bp-long DC5 fragment was found to be responsible for eliciting the lens-specific activity. Mutational analysis of the DC5 fragment identified two contiguous, interdependent positive elements and a negative element which overlaps the 3'-located positive element. Previously identified ubiquitous factors delta EF1 bound to the negative element and repressed the enhancer activity in nonlens cells. Mutation and cotransfection analyses indicated the existence of an activator which counteracts the action of delta EF1 in lens cells, probably through binding site competition. We also found a group of nuclear factors, collectively called delta EF2, which bound to the 5'-located positive element. delta EF2a and -b were the major species in lens cells, whereas delta EF2c and -d predominated in nonlens cells. These delta EF2 proteins probably cooperate with factors bound to the 3'-located element in activation in lens cells and repression in nonlens cells. delta EF2 proteins also bound to a promoter sequence of the gamma F-crystallin gene, suggesting that delta EF2 proteins are involved in lens-specific regulation of various crystallin classes. |
| 巻・号 | 13(9) |
| ページ | 5206-15 |
| 公開日 | 1993-9-1 |
| DOI | 10.1128/mcb.13.9.5206-5215.1993 |
| 解説 | Original paper. |
| PMID | 8355679 |
| PMC | PMC360209 |
| MeSH | Animals Base Sequence Chick Embryo Chickens DNA-Binding Proteins / metabolism Enhancer Elements, Genetic Gene Expression Regulation* Lens, Crystalline / metabolism* Mice Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins / metabolism Oligodeoxyribonucleotides / chemistry Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid* Repressor Proteins / metabolism Structure-Activity Relationship Tissue Distribution Transcriptional Activation Transfection |
| IF | 3.611 |
| 引用数 | 95 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | delta51-LucII (RDB08068) |