| RRC ID |
27302
|
| Author |
Erhard M, von Döhren H, Jungblut P.
|
| Title |
Rapid typing and elucidation of new secondary metabolites of intact cyanobacteria using MALDI-TOF mass spectrometry.
|
| Journal |
Nat Biotechnol
|
| Abstract |
Toxic cyanobacterial blooms are a threat because of secondary metabolite production. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to identify intact microorganisms. Microgram quantities of prepared cells, including solvent (acetonitrile and ethanol) and alpha-cyano-4-hydroxycinnamic acid matrix, display spectra showing predominantly the secondary metabolites including known microcystins, micropeptin, and anabaenopeptolin. A new cyclic anabaenopeptolin has been identified using the Post-Source-Decay mode. Strains of various origins can easily be typed according to their cyclic peptide production, and toxic and nontoxic algal blooms can be differentiated within minutes.
|
| Volume |
15(9)
|
| Pages |
906-9
|
| Published |
1997-9-1
|
| DOI |
10.1038/nbt0997-906
|
| PMID |
9306409
|
| MeSH |
Anabaena / metabolism
Bacterial Proteins / analysis*
Cyanobacteria / metabolism*
Microcystins
Peptides, Cyclic / analysis*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
|
| IF |
36.553
|
| Times Cited |
124
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| Algae |
NIES-90 |
